Rabbit monoclonal anti mhc 2 antibodies

The rabbit anti–SARS-CoV-2 S polyclonal antibody, and SARS-CoV-2 full-length S, S2, RBD, and variant proteins (293T cell expressed), were purchased from Sino Biologicals. Mouse monoclonal anti–β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and rabbit monoclonal anti-MHC II antibodies were purchased from Millipore. The rabbit monoclonal anti-Na/K ATPase was obtained from ABcam. The mouse monoclonal anti-SERCA2 and rabbit monoclonal anti-MHC I antibodies were obtained from Invitrogen. The rabbit monoclonal anti-BiP/GRP78, anti-XBP1, and anti–p-eIF2α antibodies were purchased from ABclonal. All commercial antibodies were validated for specificity by companies and us via Western blot. To obtain the deglycosylated protein, S, RBD, or S2 protein was deglycosylated in a buffer solution with PNGase F (Sigma) at 37 °C for 24 h in the dark. After deglycosylation, samples were purified and checked by Western blot (9 (link)).

Mouse monoclonal anti-HA, anti-NA, and anti-NP antibodies were obtained from Sino Biological. Mouse monoclonal anti-β-actin and rabbit monoclonal anti-MHCII antibodies were purchased from Millipore. Goat polyclonal anti-M1 was purchased from Santa Cruz Biotechnology. Mouse monoclonal anti-M2 antibody was obtained from ABcan. Rabbit polyclonal anti-granzyme B antibody was purchased from Aviva Systems Biology. Rabbit monoclonal anti-MHC I antibody was obtained from Invitrogen. All commercial antibodies were validated for specificity by companies and us via western blot.