Nos gal4

Manufactured by BD

Nos-GAL4 is a genetic tool commonly used in the field of neuroscience research. It is a driver line that expresses the yeast transcriptional activator GAL4 specifically in neurons expressing nitric oxide synthase (NOS) in various model organisms, such as Drosophila. This tool allows for the targeted manipulation and analysis of NOS-expressing neurons.

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Lab products found in correlation

Uas cd8 mcherry, by BD (2 mentions) Tub gal80ts, by BD (1 mentions) Tubulin gal4, by BD (1 mentions) Uas egfp, by BD (1 mentions) Nubbin gal4, by BD (1 mentions)

5 protocols using nos gal4

Drosophila Neuron Genetic Toolkit

Cited 3 times

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Stocks from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. Previously described fly strains: UAS-CD8-mCherry (BDSC# 27392); nos-GAL4 (BDSC # 25394) (Tracey et al. 2000 (link)); UAS-DSCP-B2 (Williams et al. 2019 (link)); LH2094 VT006486-p65ADZp (attP40); VT008489-ZpGDBD (attP2) (Dolan et al. 2019 (link)); MBON-6 MB434B R30E08-p65ADzp (attP40); R53C10-ZpGdbd (attP2); LH1900 R17A04-p65ADZp (attP40); VT041432-ZpGDBD (attP2) (Aso et al. 2014 (link), Dolan et al. 2019 (link)); SS02702 R67A06-p65ADZp (attP40); R11F03-ZpGdbd (attP2) (Robie et al. 2017 (link)); LPi4-3 R38G02-p65ADZp (attP40); R24A07-ZpGdbd (attP2) (Klapoetke et al. 2017 (link)); LPLC1 R64G09-p65ADZp (attP40); R37H04-ZpGAL4DBD (attP2) (Wu et al. 2016 (link)); MBON-22 R64F07-p65ADZp (su(Hw)attP8); R57C10-ZpGdbd (attP2) (Aso et al. 2014 (link)).

Tison K.V., McKinney H.M, & Stowers R.S. (2019). Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using Drosophila vAChT. G3: Genes|Genomes|Genetics, 10(2), 495-504.

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Drosophila Genetic Tools Database

Cited 7 times

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Stocks from the Bloomington Drosophila Stock Certer (NIH P40OD018537) were used in this study. Previously described fly strains: 20XUAS-DSCP-B2 and B2RT-STOP-B2RT-GFP-Rab3 (Williams et al. 2019 (link)); FRT-STOP-FRT-7XMYC-vAChT and 20XUAS-DSCP-FLP (Tison et al. 2020 (link)); 20XUAS-DSCP-R (Sherer et al. 2020 (link)); B2RT-STOP-B2RT-smFLAG-vGlut (Certel et al. 2022 ); vGAT¹ (Fei et al. 2010 (link)); UAS-CD8-mCherry (BDSC #27392); nos-GAL4 (Tracey et al. 2000 (link)) (BDSC #4442); MBON-6/MB434B (Aso et al. 2014 (link)); LH2094 (Dolan et al. 2019 ); 3XUAS-Syt-sm-HA (Wolff and Rubin 2018 (link)); SS02704, SS02714, SS02766, SS02705, SS02767, SS02702, SS07206, SS02710, SS02709, SS02712, SS02765, and SS02769 (Robie et al. 2017 (link)); SS00238 (Turner-Evans et al. 2020 (link)); SS46209, SS47082, SS30377 (Sterne et al. 2021 ). The 20XUAS-DSCP-R plasmid (Sherer et al. 2020 (link)) was inserted at landing site JMK66B (Knapp et al. 2015 (link)).

Certel S.J., McCabe B.D, & Stowers R.S. (2022). A Conditional GABAergic Synaptic Vesicle Marker for Drosophila. Journal of neuroscience methods, 372, 109540.

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Drosophila Gametogenesis and Cell Biology

Cited 3 times

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tubulin-Gal4 (BDSC#5138), UAS-PLCδ-PH-EGFP (BDSC#39693), tGPH (BDSC#8163), Sqh-GFP.RLC (BDSC#57145), Sqh-mCherry.M (BDSC#59024), p(Ubi-p63E-Feo-mCherry)3 (BDSC#59277), bam-Gal4 [51 (link)], nos-Gal4 (BDSC#4937), chif-Gal4 (BDSC#13134), C587-Gal4 (BDSC#67747), EYFP-Rab4 (BDSC#62542), EYFP-Rab6 (BDSC#62544), EYFP-Rab7 (BDSC#62545), EYFP-Rab11(BDSC#62549), UAS-EGFP (BDSC#5430), tub-Gal80ts (BDSC#7108), UASP-Rab11.S25N (BDSC#23261), UAS-mRFP-Anillin (BDSC#52220), rab7 mutant [69 (link)], Rab35 mutant [71 (link)], genomic CPES_V5tag [27 (link)], cpes mutants [27 (link)], UAS-hSMS1 [27 (link)], dcert1[43 (link)], UAS CDase [44 (link)], UAS-CPES active site mutants, UAS-Aedes CPES, UAS-Bombyx CPES (this study).

Kunduri G., Le S.H., Baena V., Vijaykrishna N., Harned A., Nagashima K., Blankenberg D., Yoshihiro I., Narayan K., Bamba T., Acharya U, & Acharya J.K. (2022). Delivery of ceramide phosphoethanolamine lipids to the cleavage furrow through the endocytic pathway is essential for male meiotic cytokinesis. PLoS Biology, 20(9), e3001599.

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Drosophila Genetics Experimental Protocols

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All flies were cultured on standard corn meal food at 25°C. Information for alleles and transgenes used in this study can be found either in FlyBase or as noted: Nos-Gal4 (BDSC, #4937), Tj-Gal4 (DGRC, #104055), Upd-Gal4 (BDSC, #26796), MS1096-Gal4 (THFC, #TB00038), CHES-1-like RNAi (THFC, #THU2388), Dpp-lacZ, Dad-lacZ, BMPGFP/+, and Bam-Gal4;Δ86/+ are gifts from DH Chen.

Yu J., Liu Y., Lan X., Wu H., Wen Y., Zhou Z., Hu Z., Sha J., Guo X, & Tong C. (2016). CHES-1-like, the ortholog of a non-obstructive azoospermia-associated gene, blocks germline stem cell differentiation by upregulating Dpp expression in Drosophila testis. Oncotarget, 7(27), 42303-42313.

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Genetic Toolbox for Drosophila Mitochondrial Research

Cited 2 times

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All fly stocks were raised on standard media at 25°C unless otherwise stated. Lines used in this study include nos-Cas9 (BDSC:54591), nubbin-GAL4 (BDSC:42699), nos-GAL4 (BDSC:64308), Ubi-mtSSB-GFP (a gift from Patrick O’Farrell, University of California, San Francisco), Opa1-3HA (Liu et al., 2020 (link)), mt:ND2^del1+CoI^T300I (Ma et al., 2014 (link)), D. melanogaster (mt:mauritiana; Ma and O’Farrell, 2016 (link)), w1118 and w*;attP40. REC-Halo, REC-FLAG, REC^ΔMTS-Halo, REC^KO-Halo, HDM-Halo, PolG1-Halo, XLF1(CG12728)-Halo, SPN-B-Halo, SPN-D-Halo, MRE11-Halo, NBS-Halo, UASp-REC-GFP, UASp-mito-NciI, and mt:NciI^resistant were generated for this paper.

Klucnika A., Mu P., Jezek J., McCormack M., Di Y., Bradshaw C.R, & Ma H. (2022). REC drives recombination to repair double-strand breaks in animal mtDNA. The Journal of Cell Biology, 222(1), e202201137.

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