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Mcp 1

Manufactured by Mesoscale
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Sourced in United States

The MCP-1 is a compact, high-performance microplate centrifuge designed for a variety of laboratory applications. It features a brushless motor and a high-strength aluminum rotor that can accommodate up to 8 microplates or 32 microtubes. The MCP-1 operates at a maximum speed of 4,000 rpm and provides a maximum RCF of 2,722 x g, making it suitable for numerous centrifugation tasks in the laboratory.

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Lab products found in correlation

Tnf α, by Mesoscale (2 mentions) Mip1α, by Mesoscale (2 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (2 mentions) Interleukin 6 (il 6), by Mesoscale (1 mentions) Il 12p70, by Mesoscale (1 mentions) Mcp 1, by BioLegend (1 mentions) Rantes, by BioLegend (1 mentions) Tgfb1, by R&D Systems (1 mentions) Gm csf, by Mesoscale (1 mentions)

Close spelling variants of this product

V-PLEX Human MCP-1 kit Human MCP-1 Ultra-Sensitive kit Mouse MCP-1 single-plex

5 protocols using mcp 1

1

Measuring Neuroinflammatory Markers in ART-Naïve Individuals

Cited 1 time
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We selected monocyte and macrophage activation markers, as well as specific inflammatory markers that have previously been associated with NCI in ART-naïve individuals35 (link)36 (link)37 (link). Levels of selected monocyte activation and inflammatory markers were measured in CSF and blood plasma by enzyme-linked immunosorbent assay (ELISA): sCD163 (Trillium Diagnostics, Brewer, ME, USA); or by electrochemiluminescence multiplex assay: MCP-1, IL-8, IL-6 and TNF-α, (Meso Scale Diagnostics, Rockville, MD, USA), according to the manufacturer’s procedures.
Oliveira M.F., Murrel B., Pérez-Santiago J., Vargas M., Ellis R.J., Letendre S., Grant I., Smith D.M., Woods S.P, & Gianella S. (2015). Circulating HIV DNA Correlates With Neurocognitive Impairment in Older HIV-infected Adults on Suppressive ART. Scientific Reports, 5, 17094.
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2

Quantifying Inflammatory and Angiogenic Biomarkers

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Inflammatory biomarkers and angiogenic biomarkers were quantified by ELISA. Custom multiplex assays were performed to measure mouse pro-inflammatory markers (IL-1β, IL-6, KC, IL-10, TNFa) and cytokines (MCP-1, MIP1α) (MesoScale Discovery, Gaithersburg, USA) in serum samples (diluted two-fold and four-fold, respectively). Mean reported intra- and inter-assay coefficients of variation for these assays are all <10% and <12%, respectively. Angiogenic markers (VCAM, VEGF) (R&D Systems Inc, Minneapolis, USA) were measured in serum samples and fracture callus tissue lysates (diluted 50-fold and 20-fold for measurement of VCAM, respectively and diluted fivefold for measurement of VEGF). Mean reported intra- and inter-assay coefficients of variation for these assays are all <10% and <12%, respectively.
Huang R., Vi L., Zong X, & Baht G.S. (2020). Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(10), 13521-13532.
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3

Quantifying Inflammatory and Angiogenic Biomarkers

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Inflammatory biomarkers and angiogenic biomarkers were quantified by ELISA. Custom multiplex assays were performed to measure mouse pro-inflammatory markers (IL-1β, IL-6, KC, IL-10, TNFa) and cytokines (MCP-1, MIP1α) (MesoScale Discovery, Gaithersburg, USA) in serum samples (diluted two-fold and four-fold, respectively). Mean reported intra- and inter-assay coefficients of variation for these assays are all <10% and <12%, respectively. Angiogenic markers (VCAM, VEGF) (R&D Systems Inc, Minneapolis, USA) were measured in serum samples and fracture callus tissue lysates (diluted 50-fold and 20-fold for measurement of VCAM, respectively and diluted fivefold for measurement of VEGF). Mean reported intra- and inter-assay coefficients of variation for these assays are all <10% and <12%, respectively.
Huang R., Vi L., Zong X, & Baht G.S. (2020). Maresin 1 resolves aged-associated macrophage inflammation to improve bone regeneration. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(10), 13521-13532.
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4

Multiplex Cytokine and Chemokine Analysis

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U-Plex 10 Assay Kits for analysis of IL-1b, IL-6, IL-10, IL-12p70, TNF-α, VEGFa, IP10, MCP1, TARC, and MDC were purchased from Meso Scale Diagnostics (MSD, USA). The assay was performed as described by the manufacturer. ELISA kits to measure MMP1 (DY901B), MIP1-a (DY270), MIP1-b (DY271), and IL-2 (DY202) were obtained from R&D Systems. The supernatants were tested according to the manufacturer's instructions in 96-well half area plates using 50 μL sample/test.
Tosiek M.J., Groesser K., Pekcec A., Zwirek M., Murugesan G, & Borges E. (2022). Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response. Journal of Immunology Research, 2022, 9926305.
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5

Cytokine Quantification by ELISA

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Cytokines were quantified by enzyme-linked immunosorbent assay according to the manufacturer's protocol: human IL-6, IL-2, RANTES, MCP-1 (Biolegend) and TGFb1 (R&D Systems, Minneapolis, MN). Supernatants were acidified and neutralized to activate latent TGFb1. Supernatants were also tested in the human RANTES 384 Tissue Culture Kit and the U-PLEX Human assay for IL-6, IL-10, MCP-1, and GM-CSF (Meso Scale Discovery, Rockville, MD).
Cross A.R., Lion J., Poussin K., Assayag M., Taupin J.L., Glotz D, & Mooney N. (2019). HLA-DQ alloantibodies directly activate the endothelium and compromise differentiation of FoxP3high regulatory T lymphocytes. Kidney international, 96(3).
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