ATP concentration was measured in a luminometer (Lumi-Scint, Bioscan) by the luciferin/luciferase chemiluminescent method48 . In both cases, 5 mM pyruvate +2,5 mM malate or 20 mM succinate were used as respiring substrates to assess the activity of the pathway formed by Complexes I, III and IV and the pathway composed by Complex II, III and IV, respectively.
Lumi scint
Lumi-Scint is a high-performance luminescence detection system designed for sensitive and accurate measurement of luminescent samples. The system utilizes a photomultiplier tube (PMT) detector to capture and quantify light emissions from various types of luminescent assays.
6 protocols using lumi scint
Mitochondrial Respiration and ATP Measurement
ATP concentration was measured in a luminometer (Lumi-Scint, Bioscan) by the luciferin/luciferase chemiluminescent method48 . In both cases, 5 mM pyruvate +2,5 mM malate or 20 mM succinate were used as respiring substrates to assess the activity of the pathway formed by Complexes I, III and IV and the pathway composed by Complex II, III and IV, respectively.
Quantitative Measurement of ATP
ATP Synthesis in Rod Outer Segments
Measuring ATP Synthesis in CT26 Cells
Luminescent Measurement of Mitochondrial ATP Synthesis
ATP Synthesis in Rod Outer Segments
When necessary, isolated rod OS were preincubated with 30 µM RV for 15 minutes or 5 mM MTF for 2 hours.
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