Mouse spermatogenic GC-1 spg cell line was purchased from the American Tissue Culture Collection (Shanghai, China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen, Waltham, MA, USA) containing 10% fatal bovine serum (Invitrogen) at 37°C with 5% CO2. To establish a DITD model in vitro, GC-1 spg cells were stimulated by 30 mmol/L of glucose (Invitrogen) for 24 hours. Small interfering (si) RNAs targeting METTL3 (si-METTL3), serine and arginine rich splicing factor 1 (SRSF1; si-SRSF1), pcDNA3.1-METTL3, pcDNA3.1-TUG1, and corresponding scrambled controls were purchased from GenePharma. Target plasmid vectors or negative controls were transfected to GC-1 spg cells using Lipofectamine 3000 (Invitrogen).
Si srsf1
Manufactured by GenePharma
Sourced in China
The Si-SRSF1 is a laboratory equipment designed for the knockdown of SRSF1 gene expression. It utilizes small interfering RNA (siRNA) technology to target and silence the SRSF1 gene, which plays a role in the regulation of RNA splicing. The core function of the Si-SRSF1 is to provide a tool for researchers to investigate the effects of SRSF1 knockdown in various experimental models and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fatal bovine serum, by Thermo Fisher Scientific (1 mentions)
Glucose, by Thermo Fisher Scientific (1 mentions)
Si mettl3, by GenePharma (1 mentions)
Pcdna3.1 mettl3, by GenePharma (1 mentions)
Pcdna3.1 tug1, by GenePharma (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Ccl21, by ZSGB-BIO (1 mentions)
Si malat1 1, by GenePharma (1 mentions)
Si malat1 2, by GenePharma (1 mentions)
Lipofectaminetm 2000, by Thermo Fisher Scientific (1 mentions)
Sgc 7901, by American Type Culture Collection (1 mentions)
2 protocols using si srsf1
1
In vitro DITD Model Establishment
2
GC Cell Line Transfection and Maintenance
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Human GC cell line SGC-7901 (American Type Culture Collection, ATCC, Manassas, VA, USA) was maintained in the Roswell Park Memorial Institute (RPMI)-1640 (South Logan, UT, USA) with 10% fetal bovine serum (Tianhang Biotechnology Co., Ltd., Zhejiang, China) and 100 units/ml penicillin/streptomycin in an incubator with 5% CO2 at 37 °C. The medium was renewed every 24—48 h. The cells exhibiting logarithmic growth were subsequently detached using 0.25% trypsin and transfected with CCL21 (150 μg·L−1 CCL21, ZSGB-Bio, Beijing, China), oe-MALAT1-1, si-MALAT1-1, si-MALAT1-2, or si-SRSF1 (the above plasmids designed, synthesized and constructed by Shanghai GenePharma Co. Ltd., Shanghai, China) in accordance with the instructions of the LipofectamineTM2000 (Invitrogen, Carlsbad, CA, USA).
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