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Pka inhibitor h89

Manufactured by Beyotime
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PKA inhibitor H89 is a chemical compound that functions as a potent and selective inhibitor of cAMP-dependent protein kinase (PKA). It suppresses PKA activity by competing with ATP for the catalytic site of the enzyme.

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Lab products found in correlation

Hek293 cell, by American Type Culture Collection (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Raw 264.7 cells, by American Type Culture Collection (1 mentions) 8 pcpt 2 o me camp, by Biolog (1 mentions) 6 bnz camp, by Biolog (1 mentions) Roflumilast, by Cayman Chemical (1 mentions) Mip 1α, by R&D Systems (1 mentions) Mip 1β, by R&D Systems (1 mentions) Tnf α, by R&D Systems (1 mentions) Mip 2, by R&D Systems (1 mentions) Rolipram, by Cayman Chemical (1 mentions) Tgf β1, by Thermo Fisher Scientific (1 mentions) Cbp creb interaction inhibitor, by Merck Group (1 mentions) 4 hydroxytamoxifen 4 oh, by Merck Group (1 mentions) Forskolin, by Selleck Chemicals (1 mentions) Tgf β1, by Selleck Chemicals (1 mentions) Pcdna3, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Anti phospho creb, by Cell Signaling Technology (1 mentions)

3 protocols using pka inhibitor h89

1

Regulations of Inflammatory Cytokine Secretion

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RAW264.7 cells and HEK293 cells are obtained from ATCC, and maintained in DMEM (Dulbecco′s modified Eagle′s medium) supplemented with 10% fetal bovine serum (FBS) from Gibico. BSA (bovine serum albumin) and anti-BSA antibody (α-BSA) are obtained from Invitrogen and MP Biomedicals, respectively. Dimethyl sulfoxide (DMSO) is obtained from Sigma-Aldrich. Rolipram and Roflumilast are obtained from Cayman Chemical and APExBIO, respectively. 6-Bnz-cAMP (PKA agonist) and 8-pCPT-2′-O-Me-cAMP (Epac agonist) are obtained from Biolog Life Science Institute. PKA inhibitor H-89 is obtained from Beyotime Biotechnology. ELISA kits for measuring cAMP, TNF-α, MIP-1α, MIP-1β, MIP-2 and KC are all obtained from R&D Systems. ELISA kit for measuring mouse albumin is obtained from Bethyl Laboratories.
Yan C., Chen J., Tang H., Deng C., Zhang Q, & Wang X. (2023). IgG immune complex-induced acute lung injury is ameliorated by cAMP via down-regulation of C/EBP- and AP-1-mediated transcriptions. Journal of Inflammation (London, England), 20, 34.
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2

Isolation and Culture of Murine Femoral Head Cartilage

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Femoral head cartilage and chondrocytes were isolated from 4-week-old mice as previously described16 (link),17 (link). At least three femoral head cartilage were pooled as one sample.
Femoral head cartilage isolated from Alk5Col2ERT2 and Cre-negative mice was cultured in tissue culture method and treated with 1 μM 4-hydroxy tamoxifen (4-OH TM) (Sigma–Aldrich) for 72 h to drive Cre-mediated recombination and delete Alk5, then treated with TGF-β1 (Peprotech) or forskolin (Selleck) for indicated time.
Femoral head cartilage isolated from Prg4GFPCreERT2/+ mice was treated with TGF-β1 with/without other chemical compounds, ALK5 inhibitor SB-505124 (Selleck), PKA inhibitor H89 (Beyotime), CBP-CREB interaction inhibitor (Millipore), for indicated experiments.
Primary chondrocytes were cultured in monolayer cell culture method and used in transfection and phosphorylation experiments.
Wang Q., Tan Q.Y., Xu W., Qi H.B., Chen D., Zhou S., Ni Z.H., Kuang L., Guo J.Y., Huang J.L., Wang X.X., Wang Z.Q., Su N., Chen L., Chen B., Jiang W.L., Gao Y., Chen H.G., Du X.L., Xie Y.L, & Chen L. (2017). Cartilage-specific deletion of Alk5 gene results in a progressive osteoarthritis-like phenotype in mice. Osteoarthritis and cartilage, 25(11), 1868-1879.
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3

Investigating kappa-opioid receptor signaling

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Human Dyn A(1-13) and BK were obtained from Phoenix Pharmaceuticals (Belmont, USA). The mammalian expression vector pcDNA3.1(+) and Lipofectamine™ 2000 were obtained from Invitrogen (Paisley, UK). Forskolin (FSK), 3-isobutyl-1-methylxanthine (IBMX), the Duolink PLA and protein kinase C (PKC) inhibitor Bisindolylmaleimide II (BisII) were all purchased from Sigma-Aldrich Shanghai Trading Co Ltd (Shanghai, China). PKA inhibitor H89 was purchased from Beyotime (Shanghai,China). Anti-KOR and anti-B2R antibodies were purchased from Novus (Littleton, USA). Anti-phospho-CREB and anti-total CREB antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).
Ji B., Liu H., Zhang R., Jiang Y., Wang C., Li S., Chen J, & Bai B. (2017). Novel signaling of dynorphin at κ-opioid receptor/bradykinin B2 receptor heterodimers. Cellular signalling, 31.
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