The largest database of trusted experimental protocols

2 protocols using mouse il 1β simplex

1

Mitochondrial Dynamics in Immune Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
TNF was from R&D Systems. LPS (L2630), Antimycin A, CCCP, Rotenone, Mito-TEMPO, bafilomycin A1, MG132, and CHX were from Sigma-Aldrich. ATP was from ENZO Life Sciences. TMRE, MitoTracker Deep Red, MitoTracker Green and MitoSox Red were from Life technologies. HBSS was from Cellgro. The ProcartaPlex Mouse Basic kit, mouse IL-1β Simplex and IL-18 Simplex were from eBioscience. Monoclonal antibodies used in this study are listed in Supplementary Table 1. The mCherry-Parkin plasmid was a gift from Richard Youle (Addgene plasmid # 23956)1 (link),2 (link). The following siRNA oligos were from Thermo Scientific (Dharmacon products): control siRNA (D-001210-02); ARF siRNA # 1 (5′-AGGUGAUGAUGAUGGGCAATT-3′); ARF siRNA # 2 (5′-GGUCGCAGGUUCUUGGUCATT-3′); p62 siRNA (L-047628-01); Jnk2 siRNA (5′-CCGCAGAGUUCAUGAAGAATT-3′); Jnk1 siRNA (5′-UGAUUCAGAUGGAGUUAGATT-3′); Beclin 1 (5′-CAGUUUGGCACAAUCAAUATT-3′); ATG5 siRNA (5′-CAUCAACCGGAAACUCAUTT-3′).
+ Open protocol
+ Expand
2

Mitochondrial Dynamics in Immune Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
TNF was from R&D Systems. LPS (L2630), Antimycin A, CCCP, Rotenone, Mito-TEMPO, bafilomycin A1, MG132, and CHX were from Sigma-Aldrich. ATP was from ENZO Life Sciences. TMRE, MitoTracker Deep Red, MitoTracker Green and MitoSox Red were from Life technologies. HBSS was from Cellgro. The ProcartaPlex Mouse Basic kit, mouse IL-1β Simplex and IL-18 Simplex were from eBioscience. Monoclonal antibodies used in this study are listed in Supplementary Table 1. The mCherry-Parkin plasmid was a gift from Richard Youle (Addgene plasmid # 23956)1 (link),2 (link). The following siRNA oligos were from Thermo Scientific (Dharmacon products): control siRNA (D-001210-02); ARF siRNA # 1 (5′-AGGUGAUGAUGAUGGGCAATT-3′); ARF siRNA # 2 (5′-GGUCGCAGGUUCUUGGUCATT-3′); p62 siRNA (L-047628-01); Jnk2 siRNA (5′-CCGCAGAGUUCAUGAAGAATT-3′); Jnk1 siRNA (5′-UGAUUCAGAUGGAGUUAGATT-3′); Beclin 1 (5′-CAGUUUGGCACAAUCAAUATT-3′); ATG5 siRNA (5′-CAUCAACCGGAAACUCAUTT-3′).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!