Py783 plc γ1

Manufactured by Cell Signaling Technology

PY783 PLC-γ1 is a product offered by Cell Signaling Technology. It is a laboratory reagent that detects phosphorylated phospholipase C gamma 1 (PLC-γ1) by immunoblotting. PLC-γ1 is an important enzyme involved in intracellular signal transduction pathways.

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Lab products found in correlation

Plcγ1, by Cell Signaling Technology (2 mentions) Slp 76 py128, by BD (2 mentions) Grb2 clone 23, by Santa Cruz Biotechnology (2 mentions) Actin clone c4, by Merck Group (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Slp 76 clone f 7, by Santa Cruz Biotechnology (1 mentions) Pthr308 akt, by Cell Signaling Technology (1 mentions) Precast polyacrylamide gel, by Bio-Rad (1 mentions) Src py416, by Cell Signaling Technology (1 mentions) Criterion polyacrylamide gel, by Bio-Rad (1 mentions) Sea block buffer, by Thermo Fisher Scientific (1 mentions) Lat py226, by BD (1 mentions) P38 pt180 py182, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Santa Cruz Biotechnology (1 mentions)

2 protocols using py783 plc γ1

Immunoblotting of Signaling Proteins

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Samples containing proteins were loaded onto a 4–15% precast polyacrylamide gels (Biorad). The separated proteins were transferred onto PVDF membranes (Millipore), and then blocked for 1 hr at room temperature in a 1:1 1XPBS:SEA Block (Thermo Scientific). The PVDF membranes were incubated with primary antibodies against SLP-76 pY128 (clone J141-668.36.58, BD Pharmingen), SLP-76 (clone F-7, Santa Cruz Biotechnology), AKT pThr 308 (Cell Signaling), GRB2 (clone 23, Santa Cruz Biotechnology), pY783 PLC-γ1 (Cell signaling), PLC-γ1 (Cell Signaling), pY (4G10, Millipore), Actin (clone C4, Millipore), or GAPDH (Meridian Life Sciences). IRDye 800CW or IRDye 680-conjugated secondary antibodies were diluted in SEA Block as above and incubated with the PVDF membranes for 30 min at room temperature. The membranes were then imaged using the Licor Odyssey Infrared detector.

Bilal M.Y., Zhang E.Y., Dinkel B., Hardy D., Yankee T.M, & Houtman J.C. (2015). GADS is Required for TCR-Mediated Calcium Influx and Cytokine Release, but not Cellular Adhesion, in Human T Cells. Cellular signalling, 27(4), 841-850.

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Quantitative Immunoblotting of Cell Signaling

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Protein containing samples (equivalent of 5 × 10⁶ cells) were loaded onto a 4–15% precast Criterion polyacrylamide gel (Biorad). The separated proteins were transferred onto PVDF membranes (Millipore), and then blocked for 1 h at room temperature in a 1:1 1XPBS:SEA Block buffer (Thermo Scientific). The PVDF membranes were then incubated with primary antibodies against GRB2 (clone 23, Santa Cruz Biotechnology), LAT pY226 (clone J96-1238.58.93, BD Pharmingen), LAT pY132 (Genetex), SLP-76 pY128 (clone J141-668.36.58, BD Pharmingen), ERK1/ERK2 pY187/pT185 (Invitrogen), p38 pT180/pY182 (Cell Signaling), JNK pT183/pY195 (Cell Signaling), Akt pS473 (clone-14-6, Invitrogen), Src pY416 (Cell Signaling), pY783 PLC-γ1 (Cell signaling), PLC-γ1 (Cell Signaling), lymphocyte-specific protein tyrosine kinase (LCK) (Cell Signaling), pY (4G10, Millipore), Gsk3αβ pS21/9 (Cell Signaling), actin (clone C4, Millipore), or GAPDH (Meridian Life Sciences). Secondary antibodies conjugated to IRDye 800CW or IRDye 680 were diluted in SEA Block and incubated with the PVDF membranes for 30 min at room temperature. The membranes were then visualized using the Licor Odyssey Infrared detector.

Bilal M.Y, & Houtman J.C. (2015). GRB2 Nucleates T Cell Receptor-Mediated LAT Clusters That Control PLC-γ1 Activation and Cytokine Production. Frontiers in Immunology, 6, 141.

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