Ripa buffer high r0010

Reagent grade ICA (purity ≥ 98% by HPLC analysis) was obtained from Nanjing Zelang Medical Technology Corporation Ltd. (Nanjing, China) and dissolved in dimethyl sulfoxide (DMSO); the final concentration of DMSO in the media was less than 0.1% (v/v). NaN₃ (A0639) was purchased from Amresco (Solon, OH, USA). RIPA buffer (high) (R0010) and protein phosphatase inhibitor (P1260) were purchased from Solarbio Life Science (Beijing, China). A glucose oxidase assay kit (E10160) and antibodies against GSK-3β (9315), p-Ser9-GSK-3β (9323) and p-PI3K (p85) (4228) were obtained from Cell Signaling Technology (Beverly, MA, USA). Goat anti-mouse IgG-HRP (SA00001-1), goat anti-rabbit IgG-HRP (SA00001-2), and antibodies against GAPDH (60004-1-Ig), and PI3K p110α (21890-1-AP) were obtained from Proteintech Group (Wuhan, China). Antibodies against PHF1 (ab184951) and PI3K (ab86714) were obtained from Abcam (Cambridge, MA, USA). PageRuler prestained protein ladder (26616) and antibodies against p-T217 (44-744) and TAU-5 (MA5-12808) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). RPMI 1640 HyClone™ cell culture medium (SH30809.01) was purchased from GE Healthcare (Chicago, IL, USA).

Reagent-grade ICT, (purity 99.5% by HPLC analysis) was obtained from Zhongke Quality Inspection Biotechnology Co., Ltd. (Beijing, China) and dissolved in dimethyl sulfoxide (DMSO); the final concentration of DMSO in the medium was less than 0.1% (v/v). Human TDP-43 and polybrene were purchased from Hanbio Biotechnology Co., Ltd. (Shanghai, China), a TransZol Up Plus RNA Kit and EasyScript One-Step gDNA Removal and cDNA Synthesis SuperMix were purchased from TransGen Biotechnology Co., Ltd. (Beijing, China) and NuHi Robustic SYBR Green Mix was purchased from Nuhigh Biotechnologies Co., Ltd. (Suzhou, China). High-Sig ECL Western Blotting Substrate was purchased from Tanon Technology Co., Ltd. (Shanghai, China). RIPA buffer (high) (R0010) was purchased from Solarbio Life Science (Beijing, China). A GAPDH antibody, a TDP-43 antibody, a CytC antibody, and HRP-conjugated Affinipure goat anti-rabbit IgG (H+L) were purchased from Proteintech Group (Wuhan, China). A goat anti-mouse IgG (H+L) secondary antibody was purchased from Thermo Fisher Scientific (Waltham, USA).