Mouse eyes were embedded in Tissue-Tek O.C.T. compound (Sakura Finetek, Alphen aan den Rijn, the Netherlands) and cut into sections 7 µm thick. The samples were stained with the rat anti-mouse Ly6G antibody (Abcam, Cambridge, UK) overnight at 4°C and then subsequently incubated in FITC-conjugated goat anti-rat IgG (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) for 2 hours, as previously described.24 (link) All slides were examined using fluorescence microscopy after counterstaining with 4′,6-diamidino-2-phenylindole (Vector Laboratories, Burlingame, CA, USA).
Fitc conjugated goat anti rat igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
FITC-conjugated goat anti-rat IgG is a secondary antibody that recognizes and binds to rat immunoglobulin G (IgG) molecules. The antibody is conjugated with the fluorescent dye fluorescein isothiocyanate (FITC), which can be used to detect and visualize rat IgG in various immunological applications.
Automatically generated - may contain errors
Lab products found in correlation
Rat anti mouse ly6 g antibody, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions)
Tissue tek oct compound, by Sakura Finetek (1 mentions)
Cy3 conjugated goat anti rat igg, by Thermo Fisher Scientific (1 mentions)
Cy3 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Las x software, by Leica (1 mentions)
Tcs sp8 confocal microscope, by Leica (1 mentions)
4 protocols using fitc conjugated goat anti rat igg
1
Immunofluorescence Analysis of Ly6G in Mouse Eyes
2
Visualizing LMP2A Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vac-LMP2A infected B2-1 or 143B cells and Renca-LMP2A tumor cells were fixed with 4% v/v paraformaldehyde, permeabilized with 0.1% v/v saponin solution and stained with 1:50 dilution of a rat mAb to LMP2A (14B7), followed with 1:50 dilution of FITC-conjugated goat anti-rat IgG (Invitrogen Life Technologies, Paisley, UK). Cells were visualized using 63× oil immersion lens on a confocal immunofluorescence microscope and images captured with Leica Confocal Software (Leica, Milton Keynes, UK).
3
Immunofluorescent Labeling of Brain Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Individual brain sections were incubated with PBS containing 0.3% Triton X-100 and 3% normal donkey serum for 2 hours, and then with the appropriate primary antibodies overnight for 4°C as follows: rabbit anti-Oligo2 polyclonal antibody (1:500; Millipore, Billerica, MA, USA), rat anti-MBP polyclonal antibody (1:250; Millipore) and rat anti-PLP polyclonal antibody (1:250; Millipore). The sections were washed three times in PBS and incubated with Cy3-conjugated goat anti-rabbit IgG (1:400; Invitrogen, Carlsbad, CA, USA), Cy3-conjugated goat anti-rat IgG (1:400; Invitrogen) or fluorescein isothiocyanate (FITC)-conjugated goat anti-rat IgG (1:400; Invitrogen) for 2 hours at room temperature. Finally, the sections were observed under a fluorescence microscope.
4
Quantifying ITGB7+ Cells in Colorectal Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen sections of human colorectal adenocarcinoma tissues and adjacent normal tissues were incubated overnight with anti-human ITGB7 (20 mg/mL) purified from hybridoma FIB504.64 (ATCC HB-293) at 4 C, and then incubated with FITC-conjugated goat anti-rat IgG (2 mg/mL; Invitrogen, 31629) for 1 hour at room temperature. For b7 þ cell quantification, five random fields of the intratumoral region or the normal tissue region were photographed (20Â magnification) using a Leica TCS SP8 confocal microscope with LAS X software (Leica). The number of b7 þ cells in each field was then counted, and the average of cell number per field was calculated for each slide. All slides were counted separately by two pathologists blinded to the clinical information.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!