Absorption spectra were obtained using a UV-Vis spectrophotometer (DU 720 scanning spectrophotometer, Beckman Coulter, Inc., CA USA) and luminescent spectra were obtained using a fluorescence spectrophotometer (LS-55 fluorescence spectrophotometer, PerkinElmer, Inc., MA USA). To characterize Pdot sensing, different concentrations of glucose were added to a quartz cuvette containing the Pdot glucose transducer, and the luminescent spectra were measured once per minute for 10 min with excitation at 380 nm.
Du 720 scanning spectrophotometer
Manufactured by Beckman Coulter
Sourced in United States
The DU 720 Scanning Spectrophotometer is a laboratory instrument designed for the measurement of the absorbance or transmittance of light through a sample. It features a wavelength range of 190 to 1100 nanometers and can be used for various spectroscopic applications in a laboratory setting.
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Lab products found in correlation
2 protocols using du 720 scanning spectrophotometer
1
Characterization of Pdot Glucose Sensor
2
Characterization of Conjugated Polymer Dots
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Ultraviolet/visible absorption spectra of the Pdots solution were recorded with a DU 720 scanning spectrophotometer from Beckman Coulter, Inc. (Brea, CA, USA). Fluorescence spectra were acquired in a commercial Fluorolog-3 fluorometer from HORIBA Jobin Yvon Inc. (Edison, NJ, USA). Fluorescence lifetime measurements were carried out using a PicoQuant Fluorescence lifetime system (PicoQuant Photonics North America Inc., Westfield, MA, USA). The particle sizes of Pdots in bulk solution were measured by the dynamic light scattering (NanoS Zetasizer) from Malvern (Westborough, MA, USA). For the electron micrograph measurements, two to three drops of the Pdots solution (∼20 p.p.m.) were placed on a carbon-coated copper grid (200 mesh) provided from TED PELLA, Inc. (Redding, CA, USA) and then dried under ambient conditions. The dried Pdots' sizes were measured with a transmission electron microscope (Tecnai F20) from FEI (Hillsboro, OR, USA).
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