63 oil plan apochromat objective

Manufactured by Zeiss

The ×63 oil plan-apochromat objective is a high-performance microscope objective lens manufactured by Zeiss. It is designed to provide a high numerical aperture, which enables the collection of a large amount of light and the creation of high-resolution images. The objective is characterized by its plan-apochromatic design, which ensures excellent image flatness and color correction across the entire field of view.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 700, by Zeiss (4 mentions) Gtu 88, by Merck Group (1 mentions) Lsm 700 laser confocal microscope, by Zeiss (1 mentions)

Close spelling variants of this product

Plan-Apochromat (503 citations) Plan-Apochromat objective (157 citations) Plan-Apochromat 63×/1.40 Oil DIC M27 objective (52 citations) Plan-Apochromat 63×/1.4 oil objective (47 citations) Plan-Apochromat 63 (44 citations) Plan-Apochromat oil objective (41 citations) Plan-Apochromat 63×/1.4 oil immersion objective (25 citations) Plan-Apochromat 63×/1.40 oil objective (16 citations) Plan-Apochromat 63×/1.4 Oil DIC objective (16 citations) Plan Apochromat 63×/1.4 NA oil immersion objective (9 citations)

3 protocols using 63 oil plan apochromat objective

Golgi Fission Dynamics Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols

HeLa cells stably expressing VSVG-GFP were seeded on glass bottom dish and transfected with siRNA in some experiments. SH-5Y cells were seeded on glass bottom dish and cotransfected with VSVG-GFP and siRNA. After 24 h cells were incubated at 40°C for 18 h with the last 1 h in the presence of 10 μg/mL cycloheximide to stop further de novo protein synthesis, and transferred to 20°C for 3 h. In some experiments inhibitors were added when the incubation at 40°C initiated. Cells were then transferred to 37°C for 20 min, fixed by 4% paraformaldehyde/PBS solution for 20 min and permeabilized by 0.2% Triton X-100-containing PBS for 10 min. Cells were blocked by 1% BSA/PBS for 10 min and then stained by anti-TGN46 antibody. The fluorescent images were obtained using a confocal laser scanning microscope (LSM700, Carl Zeiss) using an ×63 oil plan-apochromat objective (numerical aperture, 1.4; Carl Zeiss). Golgi fission index was calculated according to the definition as illustrated in Figure 6C by using ImageJ software.

Okada T., Nishida S., Zhang L., Ibrahim Mohamed N.N., Wang T., Ijuin T., Kajimoto T, & Nakamura S.I. (2021). Constitutive activation of S1P receptors at the trans-Golgi network is required for surface transport carrier formation. iScience, 24(11), 103351.

+ Open protocol

+ Expand

Visualizing S1P Receptor Localization in the Golgi

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded on glass-bottom dishes and transfected with expression vectors for VSVG-GFP or GFP and S1P₁R-mCherry or S1P₂R-mCherry. After 24 h, cells were incubated at 40°C for 18 h with the last 1 h in the presence of 10 μg/mL cycloheximide. Cells were incubated at 20°C for 3 h, followed by fixation in 4% paraformaldehyde/PBS solution for 20 min and permeabilization by 0.2% Triton X-100-containing PBS for 10 min. Cells were blocked by 1% BSA/PBS for 10 min and then stained by anti-TGN46 antibody using anti-rabbit IgG Alexa 647 as a secondary antibody. The fluorescent images were obtained using a confocal laser scanning microscope (LSM700, Carl Zeiss) using an ×63 oil plan-apochromat objective (numerical aperture, 1.4; Carl Zeiss). The percentage of S1P receptor signals localized in Golgi is calculated by using ImageJ software.

+ Open protocol

+ Expand

Immunostaining of Cellular Proteins

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were cultured on No. 1.5 glass coverslips coated with poly-L-lysine. After treatments, cells were fixed and permeablized in −20°C methanol for 1 min unless stated otherwise. Cells were then blocked and immunostained as described previously (Erie et al. 2015 (link)). The following primary antibodies were used: rabbit polyclonal anti-p62 (1:1000), mouse monoclonal anti-p62 (1:1000), mouse monoclonal anti-Ub (1:1000), Rabbit monoclonal anti-K48-linked Ub (1:1000), Rabbit monoclonal anti-K63-linked Ub (1:1000), mouse monoclonal ubiquilin-2 (1:1000. Cat. #NBP2-25164SS, Novus Biologicals, Littleton, CO), rabbit polyclonal anti-proteasome 20S α+β (1:1000, Cat. #22673, Abcam), rabbit monoclonal anti-huntingtin (1:500, Cat. #109115, Abcam), mouse monoclonal γ-tubulin (1:1000, GTU-88, Cat #T5326, Sigma) and rat monoclonal anti-lysosomal associated protein 1 (Lamp1, 1:500, 1D4B, Developmental Studies Hybridoma Bank, Iowa city, IA). Immunofluorescent signals were detected using the Zeiss LSM700 laser confocal microscope with a 63× oil Plan-Apochromat objective (numerical aperture: 1.4). In all presented images, DAPI was used as a nuclear counterstain.

Huang N., Erie C., Lu M.L, & Wei J. (2017). Aberrant subcellular localization of SQSTM1/p62 contributes to increased vulnerability to proteotoxic stress recovery in Huntington’s disease. Molecular and cellular neurosciences, 88, 43-52.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!