Cobalt chloride (II) hexahydrate (ACS reagent, 98%), iron chloride (III) hexahydrate (ACS reagent, 97%), oleic acid (90%), hexane (ACS reagent, ≥98.5%), diethyl ether (anhydrous, ACS reagent, ≥99%), toluene (ACS reagent, ≥99.5%), and chloroform (for high-performance liquid chromatography, gas chromatography, and residue analysis, ≥99.9%) were purchased from Sigma-Aldrich. 1-Octadecene (90%) was purchased from Acros Organics, and sodium oleate (>97%) was purchased from TCI America. PEG, 5 kDa for ligand exchange, was procured from Sigma-Aldrich, and block copolymer polyethylene glycol-b-poly(lactic acid) PEG4.9kDa-b-PLA6.0kDa was purchased from Evonik Industries. Tetrahydrofuran (THF; 99.9% Acros Organics) was dried using sodium sulfate before use in Flash Nanoprecipitation (FNP). HA sodium salt from Streptococcus equi was purchased from Sigma-Aldrich. Bovine synovial fluid was obtained from Animal Technologies. Water was used from Milli-Q grade (Millipore, Bedford, MA).
Ha sodium salt from streptococcus equi
Manufactured by Merck Group
Sourced in United States
HA sodium salt from Streptococcus equi is a laboratory product used for research purposes. It is a purified form of hyaluronic acid, a naturally occurring polysaccharide, extracted from the bacterial strain Streptococcus equi. This product is intended for use in various research applications that require hyaluronic acid as a component or material.
Automatically generated - may contain errors
Lab products found in correlation
Sodium carbonate na2co3, by Merck Group (2 mentions)
Iron 3 chloride hexahydrate, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
Sodium oleate, by Tokyo Chemical Industry (1 mentions)
1 octadecene, by Thermo Fisher Scientific (1 mentions)
Tetrahydrofuran thf, by Thermo Fisher Scientific (1 mentions)
Milli q grade, by Merck Group (1 mentions)
Anti cd3 fitc, by BioLegend (1 mentions)
Anti cd11c apc, by BioLegend (1 mentions)
Anti cd8 pe, by BioLegend (1 mentions)
Anti cd4 apc, by BioLegend (1 mentions)
Anti cd80 pe, by BioLegend (1 mentions)
Anti cd40 pe, by BioLegend (1 mentions)
Anti cd86 pe, by BioLegend (1 mentions)
Rpmi 1640 culture medium, by Biowest (1 mentions)
Anti ia ie fitc, by BioLegend (1 mentions)
8 protocols using ha sodium salt from streptococcus equi
1
Synthesis and Characterization of Cobalt Ferrite Nanoparticles
2
Flow Cytometry Characterization of DCs and Lymphocytes
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The monoclonal antibodies for the characterization of DC and lymphocytes by flow cytometry were the following: anti-CD3 FITC, anti-CD4 APC, anti-CD8 PE, anti-CD137-biotin, anti-CD11c APC, anti-CD40-PE, anti-CD86-PE, anti-CD80-PE, anti-F4/80-Cy5 and anti-Ia/Ie-FITC from Biolegend (San Diego, CA, USA). The murine melanoma cell line B16-F10 with haplotype H-2Kb was purchased from The American Type Culture Collection, USA. RPMI-1640 culture medium was purchased from Biowest (Nuaille, France). Ge from bovine skin with a protein percentage of 78% and a water content of 8% was used. HA sodium salt from Streptococcus equi, both from SIGMA (St Louis, MO, USA) with a solubility of 5 mg/mL and an MW of ~1.5–1.8 × 106 Da was used.
3
Purification and Labeling of β-Lactoglobulin
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β-lactoglobulin was purified from whey protein isolate (Fonterra, New Zealand) and used to prepare AF. HA sodium salt from Streptococcus equi, Curcumin from Curcuma longa, Rhodamine B, Fluorescein, Acid Fuchsin, Pepsin from porcine gastric mucosa (P6887), Calcium chloride dihydrate, Sodium chloride, and gold (III) chloride trihydrate (≥99.9%) were purchased from Sigma Aldrich.
4
Biomimetic Polysaccharide-Ceramic Composites
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The HA sodium salt from Streptococcus Equi (Mw = 15–30 kDa), fatty acids, and all solvents were purchased from Sigma–Aldrich (St. Louis, MO, USA).
Silicon oxide (SiO2), calcium carbonate (CaCO3), calcium phosphate (Ca3(PO4)2), magnesium carbonate hydroxide pentahydrate ((MgCO3)4Mg(OH)2·5H2O), sodium carbonate (Na2CO3), and potassium carbonate (K2CO3) were bought from Sigma-Aldrich and used as received for bioactive glass production.
Silicon oxide (SiO2), calcium carbonate (CaCO3), calcium phosphate (Ca3(PO4)2), magnesium carbonate hydroxide pentahydrate ((MgCO3)4Mg(OH)2·5H2O), sodium carbonate (Na2CO3), and potassium carbonate (K2CO3) were bought from Sigma-Aldrich and used as received for bioactive glass production.
5
Preparation of Aristolochic Acid and Hyaluronic Acid Solutions
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Aristolochic acid was purchased from Sigma-Aldrich (St. Louis, MO, USA), dissolved in ethanol at 5 mg/ml, and stored at 4°C until needed. It was diluted in phosphate-buffered saline (PBS) when used in the pharmacological inhibition protocols. HA sodium salt from Streptococcus equi (Sigma-Aldrich) was dissolved in ultra-pure water at 2.5 mg/ml and stored at −20°C. Commercial hyaluronidase from bovine testes (Apsen, São Paulo, Brazil) was dissolved in ultra-pure water at 5 mg/ml and stored at −20°C.
6
Hydrogel Biomaterials Synthesis
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Chondroitin-4-sulfate sodium salt from bovine trachea, DS from procaine intestinal mucosa, HA sodium salt from Streptococcus equi, H sodium salt derived from bovine intestinal mucosa, and PLGA, 50:50 (number average molecular weight [Mw] 24,000–38,000 g/mol) were purchased from Sigma-Aldrich Co (St Louis, MO, USA). Acetone was procured from VWR International (Radnor, PA, USA) and tetrahydrofuran (THF) was procured from Carl Roth GmbH (Karlsruhe, Germany).
7
Bioinspired Chitosan-Hyaluronic Acid Hydrogel
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CHI (medium molecular weight) with a degree of N-deacetylation (DD) ranging from 75 to 85% and a viscosity of 200–800 cP, HA sodium salt from Streptococcus equi (Mw = 1.5–1.8 × 106 Da), dopamine hydrochloride (DN), N-(3 Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), hydrocaffeic acid (HCA), and genipin were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Prior to being used, the CHI was purified by a recrystallization process.
Citric acid monohydrate (C6H8O7·H2O), ammonium phosphate dibasic ((NH4)2HPO4), calcium nitrate tetrahydrate 99% (Ca(NO3)2·4H2O), ethanol absolute, sodium sulphate (Na2SO4) and ammonium hydroxide solution (ammonium hydrogen phosphate 98%, maximum of 33% NH3), Tris(hydroxymethyl)-aminomethane (Tris buffer), magnesium chloride hexahydrate (MgCl2·6H2O), and hydrochloric acid (HCl) were all purchase from Sigma-Aldrich. Tetraethyl orthosilicate (TEOS) 99.90%, di-Potassium hydrogen phosphate trihydrate (K2HPO4·3H2O), calcium chloride (CaCl2), potassium chloride (KCl), sodium hydrogen carbonate (NaHCO3), and sodium carbonate (Na2CO3) were purchased from Merck Chemicals (Darmstadt, Germany). Sodium chloride (NaCl), sodium hydroxide (NaOH), and glacial acetic acid were purchased from Panreac Química SLU (Barcelona, Spain).
Citric acid monohydrate (C6H8O7·H2O), ammonium phosphate dibasic ((NH4)2HPO4), calcium nitrate tetrahydrate 99% (Ca(NO3)2·4H2O), ethanol absolute, sodium sulphate (Na2SO4) and ammonium hydroxide solution (ammonium hydrogen phosphate 98%, maximum of 33% NH3), Tris(hydroxymethyl)-aminomethane (Tris buffer), magnesium chloride hexahydrate (MgCl2·6H2O), and hydrochloric acid (HCl) were all purchase from Sigma-Aldrich. Tetraethyl orthosilicate (TEOS) 99.90%, di-Potassium hydrogen phosphate trihydrate (K2HPO4·3H2O), calcium chloride (CaCl2), potassium chloride (KCl), sodium hydrogen carbonate (NaHCO3), and sodium carbonate (Na2CO3) were purchased from Merck Chemicals (Darmstadt, Germany). Sodium chloride (NaCl), sodium hydroxide (NaOH), and glacial acetic acid were purchased from Panreac Química SLU (Barcelona, Spain).
8
Silk-Based Antimicrobial Biomaterials
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Materials Raw Bombyx mori silk was obtained from Bursa Institute for Silkworm Research (Bursa, Turkey). Sodium carbonate, calcium chloride, ethanol, bromophe-nol blue, and acetonitrile were purchased from Sigma-Aldrich (Steinheim, Germany). HA sodium salt (from Streptococcus equi) and Saponin were supplied from Sigma (St.Louis, MO, USA). OLE was kindly provided from DUAG Natural Products (Izmir, Turkey) in powder form. Formic acid, Mueller-Hinton agar, Sabouraud dextrose agar, Tween 80, lecithin, folin-ciocalteu reagent, gallic acid, and acetic acid were obtained from Merck (Darmstadt, Germany). Phosphate-buffered saline (PBS) (pH 7.2) was obtained from Gibco TM (Carlsbad, CA, USA). Trolox (6-hydroxy-2,5,7,8,-tetramethylchroman-2carboxylic acid), potassium persulfate (K 2 O 8 H 8 ), and ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)) were obtained from Fluka (Buchs, Switzerland).
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