Pooled human plasma apheresis derived

All synthetic oligonucleotide primers and dual-labeled probes were purchased from Integrated DNA Technologies (Coralville, IA, USA). MBs and dual-labeled probes were prepared using HPLC purification while primers were ordered with standard desalting. Tris(hydroxymethyl)aminomethane (Tris) and bovine serum albumin (BSA) were obtained from P212121 (Ypsilanti, MI, USA). Trisodium citrate, ethylenediaminetetraacetic acid dipotassium salt dihydrate, and dehydrated plasma were ordered from Sigma-Aldrich (St. Louis, MO, USA). Pooled human plasma apheresis derived was obtained from Innovative Research (Novi, MI, USA). Sodium chloride was obtained from Fisher Scientific (Hampton, NH, USA). Eppendorf Lobind centrifuge tubes (Hamburg, Germany) were used for all extractions. An Agilent Technologies Poroshell 120 column (50 mm × 4.6 mm i.d. × 2.7 μm particle size) was used for BSA analysis. All fluorescence experiments were performed using a BioTek Synergy Hybrid H1 microplate reader. Deionized water (18.2 MΩ cm) used was obtained from a Millipore Milli-Q water purification system (Bedford, MA, USA). For BSA extraction experiments, an Agilent 1260 HPLC with a diode array detector coupled to an Agilent 6230B Accurate Mass Time of Flight (TOF) mass spectrometer with an electrospray source was used.