Anti egfr antibody d38b1

The following antibodies were used for immunostaining. Anti-EGFR antibodies (clone D38B1) were from Cell Signaling (Danvers, MA, USA). Anti–EEA1 (clone 14/EEA1) was from BD Transductions (Allschwill, Switzerland). Antibody against LRP-1 (clone 8G1) was from Genetex (Irvine, CA, USA). Fluorescently labelled secondary antibodies were purchased from Invitrogen (Carlsbad, CA, USA) (Alexa Fluor−488; −568; −647). DAPI was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The following antibodies were used for immunoblotting: anti-EGFR antibody (D38B1) were from Cell Signaling (Danvers, MA, USA), anti-LRP-1 (EPR3724) from Abcam (Cambridge, UK), anti-DNM2 (G-4) and anti-Rab5 (D-11) were from Santa Cruz Biotechnology (Dallas, TX, USA) and anti-GAPDH (clone C65) from Millipore (Darmtadt, Germany). HRP-conjugated secondary antibodies were purchased from Invitrogen (Carlsbad, CA, USA). All other reagents were of molecular biology quality.

DU145 cells were treated with 2D5 peptide at the indicated concentrations and for the indicated periods and then lysed in lysis buffer (20 mM Tris–HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% deoxycholate, and 0.1% SDS) with sonication. After centrifugation, the supernatants were subjected to SDS-PAGE and then immunoblotted. The following antibodies were used for immunoblotting: anti-Myc antibody (9E10, Sigma), anti-HA antibody (C29F4, Cell Signaling Technology), anti-EGFR antibody (D38B1, Cell Signaling Technology), anti-phospho-EGFR Y1068 antibody (Cell Signaling Technology), anti-GST antibody (Z-5, Santa Cruz Biotechnology), anti-STAT3 antibody (79D7, Cell Signaling Technology), anti-phospho-STAT3 antibody (GeneTex), anti-ERK antibody (K-23, Santa Cruz Biotechnology), anti-phospho-ERK antibody (Cell Signaling Technology), anti-β-actin antibody (AC-15, Santa Cruz Biotechnology), anti-p65 antibody (D14E12, Cell Signaling Technology), anti-phospho-p65 antibody (93H1, Cell Signaling Technology), and anti-phosphotyrosine antibody (4G10, GeneTex).