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Cell culture supplements

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Cell culture supplements are specialized products designed to support the growth and maintenance of cells in laboratory settings. These supplements provide essential nutrients, growth factors, and other components necessary for optimal cell culture performance. They are commonly used in a variety of applications, including cell-based research, drug development, and tissue engineering.

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4 protocols using cell culture supplements

1

Antibodies and Reagents for Endothelial Cell Research

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Cell culture media, serum, and cell culture supplements were purchased from ATCC, England, UK, https://www.lgcstandards-atcc.org/?geo_country=gb Millipore, LONZA; England, UK, http://www.lonza.com/ and Thermo Fisher Scientific; England, UK, http://www.thermofisher.com/uk/en/home.html. Antibodies against VE‐cadherin (CD144) (ab33168), PECAM (CD31) (ab28364), Flk‐1 (ab9530), STAT3 (ab119352), pSTAT3 (ab76315), JAK‐1 (ab47435), GAPDH (ab8245), OCT4 (ab19857), eNOS (ab66127), and QKI (ab126742) were purchased from Abcam. Antibodies against vWF (SC‐8068) and QKI (SC‐103851) were purchased from Santa Cruz; Heidelberg, Germany, https://www.scbt.com/scbt/home;jsessionid=jQ9cedHXnegG6a6cJZbpXPfwu6Z4K8PRhaZidcYh8LrI_ccsHWgp!‐97074204#. Antibodies against QKI‐5 (AB 9904) were purchased from Millipore; England, UK, http://www.merckmillipore.com/GB/en?ReferrerURL=http%3A%2F%2Fwww.bing.com%2Fsearch%3Fq%3Dmillipore%26src%3DIE‐TopResult%26FORM%3DIETR02%26conversationid%3D.
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2

Endothelial Cell Angiogenesis Assay

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Cell culture media, serum, and cell culture supplements were purchased from ATCC, USA, Merck Millipore, USA, LONZA, Switzerland and Thermo Fisher Scientific, USA. Antibodies against VE‐Cadherin (CD144) (ab33168 and STJ96234), VEGFR (ab9530), GAPDH (ab8245), OCT‐4 (ab19857 and STJ72238), KLF4 (ab72543) eNOS (ab76198), and FSTL3 (STJ112317) were purchased from Abcam, UK or St John's Laboratory, UK. Antibodies against vWF (SC‐8068) were purchased from Santa Cruz, USA. KDR (MAB3571), β‐actin (MAB8929), recombinant FSTL3 (AF1288‐F3), and Proteome Profiler Array Human Angiogenesis Array Kit (ARY007) were purchased from R&D, USA.
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3

Endothelial Cell Marker Protocol

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Cell culture media, serum, and cell culture supplements were purchased from ATCC; Manassas, VA, USA, Millipore; USA, Invitrogen; NY, USA, and PAA; North America. Antibodies against VE‐cadherin (ab33168), Flk‐1 (ab9530), VEGFA (ab51745), STAT3 (ab119351), JAG‐1 (ab7771), NOTCH‐1 (ab27526), SMA; Smooth Muscle Actin (ab5831), and SM22 (ab14106) were purchased from Abcam; Cambridge, UK. The Antibody against GAPDH (sc‐25778) was purchased from Santa Cruz. Antibodies against CD31 were purchased from ABBIOTEC; San Diego, USA (250590) and Santa Cruz; Heidelberg, Germany (sc‐1506). The secondary antibodies for immunostaining anti‐mouse Alexa 488 and anti‐rabbit Alexa 488 were purchased from Invitrogen. The secondary antibodies for Western blotting were purchased from DakoCytomation; Glostrup, Denmark and Abcam. Recombinant mouse VEGF was purchased from R and D Systems; Abingdon, UK.
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4

Vascular Smooth Muscle Cell Characterization

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Cell culture media, serum and cell culture supplements were purchased from ATCC, Millipore, Gibco and PAA. Antibodies against Calponin, Smooth Muscle 22-α (SM-22α) and Smooth Muscle-Myosin Heavy Chain II (SM-MyHCII) were purchased from Abcam. Antibodies against Smooth Muscle-α Actin (SM-αA) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purchased from Sigma Aldrich. Antibodies against HSP47 were purchased from MBL International and Millipore. Secondary antibodies for immunostaining anti-mouse Alexa488, anti-rabbit Alexa488 and anti-rabbit Alexa594 were purchased from Invitrogen, whereas secondary antibodies for Western Blotting were purchased from Dako.
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