Tetracycline

MLN4924 (HY‐10484, MedChemExpress USA) was purchased from MedChem Express and dissolved in dimethyl sulphoxide (DMSO) (Sigma‐Aldrich, St. Louis, MO, USA) to make a 10mM to 100 mM solution and stored at −80℃. In animal experiments, the drug was dissolved in 10% HP‐β‐CD (Sangon Biotech Inc, Shanghai, China). U0126 (HY‐12031, MedChemExpress USA) was purchased from MedChemExpress and dissolved in DMSO (Sigma‐Aldrich, St. Louis, MO, USA) to generate a 1 mM solution and stored at −40℃. Tenofovir (HY‐13782A, MedChemExpress USA) was purchased from MedChemExpress and dissolved in DMSO (Sigma‐Aldrich, St. Louis, MO, USA) to generate a 1 mM solution and stored at −40℃. Tetracycline (HY‐B0474, MedChemExpress USA) was purchased from MedChemExpress and dissolved in DMSO (Sigma‐Aldrich, St. Louis, MO, USA) to generate a 100mg/ml solution and stored at −40℃.

AF and antibiotics [fosfomycin (FOF); ciprofloxacin (CIP); tetracycline (TET); linezolid (LZD); chloramphenicol (CHL); levofloxacin (LVX); teicoplanin (TEC); clindamycin hydrochloride (CLI) hydro; gentamicin (GEN); vancomycin (VAN)] were purchased from the MedChemExpress company (Monmouth Junction, NJ, United States). E. faecalis ATCC 29212, S. aureus ATCC25923 and ATCC29213 were kindly provided by Juncai Luo (Tiandiren Biotech, Changsha, China). S. epidermidis RP62A and ATCC 12228 were given by Di Qu (Shanghai Medical College of Fudan University), S. aureus ATCC43300 (MRSA), Newman, and RJ-2 were given by Min Li (Renji Hospital, Shanghai Jiao Tong University School of Medicine). Other clinical strains were isolated from the wound secretion or sputum of inpatients at the Third Xiangya Hospital of Central South University. Staphylococcus spp. were grown in tryptic soy broth (TSB) broth medium (Solarbio, Shanghai, China), and E. faecalis was grown in brain heart infusion (BHI) broth medium (Solarbio, Shanghai, China) at 37°C.