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2 protocols using donkey anti rabbit igg h l alexa fluor 488 ab150073

1

Molecular Mechanisms of Neuroinflammation

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MI-2 (S7429) was purchased from Selleckchem (Houston, Texas, USA); Human recombinant MALT1 protein (hrMALT1) (ab271604) and Luxol Fast Blue (LFB) Staining Kit (ab150675) were purchased from Abcam (Cambridge, UK). Nissl Staining Solution (C0117) and Dihydroethidium (DHE) (S0063) were purchased from Beyotime (Shanghai, China). Silver Staining Kit (G1052) was purchase from Servicebio (Wuhan, China). Evans blue (DK0001) was purchase from Leagene (Beijing, China). Antibodies: p-p65 (phos-NF-κB, ab194726), Bip (ab21685), CD31 (ab64543), GFAP (ab279290), NeuN (ab104224), AQP4 (ab259318), MMP9 (ab76003), IL-1β (ab254360), ZO-1 (ab221547), Donkey Anti-Goat IgG H&L (Cy5) (ab6566), Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) (ab150075), Donkey Anti-Goat IgG H&L (Alexa Fluor 488) (ab150129), Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) and Donkey Anti-Rabbit IgG H&L (Alexa Fluor 488) (ab150073) were purchased from Abcam (Cambridge, UK). MALT1 (GB11790) and Iba-1 (GB12105) were purchased from Servicebio (Wuhan, China). CD86 (NBP2-67417) was purchased from Novus (Minneapolis, USA).
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2

Comprehensive Antibody Characterization Protocol

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Chemical reagents, such as DMSO, NaCl, SDS, and Tris base for buffer preparation were obtained from Sigma-Aldrich (St. Louis, MO). The FBS, cell culture media, and antibiotics were purchased from Invitrogen (Grand Island, NY). Antibodies against Skp2 (#2652, IB:1:2000, IHC: 1:100), HK1 (#2024, IB: 1:2000), HK2 (#2867, IB: 1:2000, IHC:1:200), p-Akt (#4060, IB: 1:1000), Akt (#4691, IB: 1:2000), p-S6 (#4858, IB: 1:4000), p-Histone H3 (Ser10) (#53348, IF: 1:200), ubiquitin (#3936, IB: 1:1000), β-actin (#4970, IB: 1:5000), p27 (#3686, IB: 1:1000), anti-rabbit IgG HRP (#7074), and anti-mouse IgG HRP (#7076) were obtained from Cell Signaling Technology, Inc. (Beverly, MA). HK2 (LS‑C404653) antibody for IHC staining was obtained from LifeSpan BioSciences, Inc. Antibodies against Ki67 (ab16667, IHC: 1: 250) and donkey anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150073, IF: 1:800) were purchased from Abcam (Cambridge, UK).
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