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NEDD4 is a protein that plays a role in the ubiquitination process, which targets proteins for degradation. It functions as an E3 ubiquitin-protein ligase, facilitating the transfer of ubiquitin to substrate proteins.

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Lab products found in correlation

Nedd4l, by Cell Signaling Technology (2 mentions) Prolong diamond, by Thermo Fisher Scientific (1 mentions) Bsa pbs, by Roche (1 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) Snap surface alexa 488, by New England Biolabs (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Lysozyme, by Agilent Technologies (1 mentions) Ha y 11, by Santa Cruz Biotechnology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) C myc 9e10, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) β catenin, by BD (1 mentions)

2 protocols using nedd4

1

SNAP-LGR5 and SNAP-FZD5 Colocalization

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HEK293T cells were grown on laminin‐coated glass coverslips. Cells were co‐transfected with 100 ng SNAP‐LGR5 or SNAP‐FZD5 and 150 ng myc‐NEDD4, HA‐NEDD4‐CS, myc‐NEDD4L, HA‐NEDD4L‐CA or pcDNA4 as a control with Fugene according to the manufacturer's instructions. After 24 h of transfection, cells were labelled with 1 μM SNAP‐Surface Alexa 488 (NEB) for 15 min at 4°C to block endocytosis. Cells were then immediately washed with fresh RPMI media and fixed in 4% paraformaldehyde. Cells were incubated with primary antibodies NEDD4 (Santa Cruz) or NEDD4L (Cell Signaling) for 1 h at RT followed by a secondary antibody conjugated to Alexa‐568 (Invitrogen) and DAPI (Sigma) in 2% BSA‐PBS (Roche). Cells were mounted in Prolong Diamond (Life technologies) and imaged using a DeltaVision Core system.
Novellasdemunt L., Kucharska A., Jamieson C., Prange‐Barczynska M., Baulies A., Antas P., van der Vaart J., Gehart H., Maurice M.M, & Li V.S. (2019). NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor. The EMBO Journal, 39(3), e102771.
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2

Multimodal Protein Analysis Protocol

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β‐catenin (610154, BD), caspase‐3 (9661L, Cell Signalling), Cyclin D1 (2978S, Cell Signalling), DVL2 (10B5) (sc‐8026, Santa Cruz), GAPDH (sc‐47724, Santa Cruz), Flag (F3165, SIGMA), HA (Y‐11) (sc‐7392, Santa Cruz), LGR4 (C‐12) (sc‐390630, Santa Cruz), Lysozyme (A0099, Dako), c‐Myc (9E10) (sc‐40, Santa Cruz), NEDD4 (sc‐25508, Santa Cruz), NEDD4L (4013S, Cell Signalling), SNAP (P9310S, NEB), Sox9 (AB5335, Millipore) and V5 (ab27671) were used in immunohistochemistry, immunoprecipitations or Western blot analysis.
Novellasdemunt L., Kucharska A., Jamieson C., Prange‐Barczynska M., Baulies A., Antas P., van der Vaart J., Gehart H., Maurice M.M, & Li V.S. (2019). NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor. The EMBO Journal, 39(3), e102771.
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