Refrigerated bath circulator

Asparagus lucidus Lindl (Cheonji Gayakcho, South Korea) extraction was performed as previously reported [28 (link), 30 ]. Freeze dryer (IlShin, Dongducheon, Korea), rotary evaporator (EYELA, Tokyo, Japan), and refrigerated bath circulator (JEIO TECH, Daejeon, Korea) were used in this study. Using 500 g of Asparagus lucidus Lindl, a total of 65.7 g lyophilized AL extract powder was formed (yield: 13.14%). The extract was dissolved in DMSO (10 mg/mL) and stored at 4 ℃ before use.

Candidate plants, the herb medicine with anti-inflammation properties, were obtained from Jeju Island in South Korea. The plants were cleaned with distilled water and then dried using a freeze dryer (iLShin, Korea). After drying, 300 g of the dried herb was extracted three times in 3 liter of distilled water with refluxing at 80°C for 8 hours. 500 ml extract was concentrated under reduced pressure at 50°C using a rotary evaporator (EYELA, Japan) connected to a refrigerated bath circulator (Jeio Tech, Korea) [8 (link)]. The decoction was filtered using muslin cloth followed by Whatman (GE Healthcare, USA) grade 1 filter paper, lyophilized using a freeze dryer (iLShin, Korea), and stored at 4°C before further use [9 (link)]. To prepare the sample for experiments, the stock of extract powder (10 mg) was dissolved in 1 ml of dimethyl sulfoxide (DMSO).

Trichosanthes kirilowii was obtained from Jeju Island in South Korea. The whole part of the plant was dried with a freeze dryer (IlShin, Dongducheon, Korea). A total of 500 g of dried TK was extracted with 7.5 L of distilled water at 60 °C for 3 h. The extract was concentrated under reduced pressure at 50 °C using a rotary evaporator (EYELA, Tokyo, Japan) connected to a refrigerated bath circulator (JEIO TECH, Daejeon, Korea). The extract was filtered using muslin cloth followed by Whatman (GE Healthcare, Chicago, IL, USA) grade 1 filter paper and lyophilized using a freeze dryer. The extract was dissolved in dimethyl sulfoxide (DMSO) (10 mg/mL) and stored at 4 °C [20 (link),21 (link)].