Calculations were performed according to:
where WT = weight of total drug added, and WF = weight of free un-entrapped drug quantified in the ultrafiltrate, respectively.
The Amicon® Ultra-4 30k Merck Millipore is a laboratory device designed for sample concentration and buffer exchange. It utilizes a centrifugal filter with a 30 kDa molecular weight cut-off membrane to separate and concentrate macromolecules such as proteins, peptides, and nucleic acids from solutions.
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