The pcDNA3.1A plasmid was from Invitrogen (Carlsbad, CA, USA). The pcDNA-chIL7, pcDNA-chIL7/H, pcDNA-VP2 (VP2 is an epitope in IBDV VP2 consisted of 122 amino acids) and pcDNA-VP2/H-IRES-chIL7/H and pcDNA-VP2-IRES-chIL7 plasmids were previously constructed in our laboratory [17 (link)]. Human embryonic kidney (HEK) 293T cells (ATCC CRL-11268) and UMNSAH/DF-1 chicken embryonic fibroblast cells (DF-1 cells) (ATCC CRL-12203) were purchased from ATCC (Manassas, VA, USA). A virulent IBDV strain (Harbin-1 strain) was kindly provided by Zandong Li of China Agricultural University. Specific pathogen–free (SPF) chickens were purchased from Jinan Sais Poultry Company (Jinan, China) and raised in an isolator in an environmental control room with a 12/12 h light/dark cycle. Animal experiments were approved by the Animal Ethics Committee of the Hebei Agricultural University (No. DW20170016)
Umnsah df 1 chicken embryonic fibroblast cells df 1 cells
Manufactured by American Type Culture Collection
Sourced in United States
UMNSAH/DF-1 chicken embryonic fibroblast cells (DF-1 cells) are a continuous cell line derived from chicken embryonic fibroblasts. DF-1 cells are commonly used as a model system for various research applications.
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Lab products found in correlation
2 protocols using umnsah df 1 chicken embryonic fibroblast cells df 1 cells
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Recombinant Plasmid Construction and Validation
2
In Vitro and In Vivo Evaluation of IBDV
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Human embryonic kidney (HEK ) 293T cells were purchased from the Cell Culture Center of Peking Union Medical University (Beijing, China). Mouse immature B lymphocytes (2E8 cells) and UMNSAH/DF-1 chicken embryonic fibroblast cells (DF-1 cells) were purchased from ATCC (Manassas, VA). The virulent IBDV strain HB-1 serotype-1 were kindly presented by Dr. Weiquan Liu in China Agricultural University. Specific pathogen–free (SPF ) chickens (21-day-old White Leghorn layers) were purchased from a local chicken farm. The chickens were kept in the specific isolator in an environmentally controlled room with a 12/12 hour light/dark cycle. Animal experiments were performed based on the Guide for the Care and Use of Laboratory Animals of the Agricultural University of Hebei.
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