Ulex europaeus 1 uea 1 lectin

Evans blue, methamphetamine (METH) and MLA were purchased from Sigma-Aldrich (St. Louis, MO). Dynabeads M-450 Tosylactivated was purchased from Invitrogen (Carlsbad, CA). Ulex europaeus I (UEA I) lectin and mounting medium with DAPI were purchased from Vector (Buringame, CA). The Gp41 ectodomain peptide (gp41-I90) was prepared as described previously [9 (link), 11 (link)]. A senescence β-Galactosidase Staining Kit (Cat#9860) was bought from Cell Signaling Technology. A Fluoro-Jade B (FJB) staining kit was obtained from Histo-Chemo Inc. All primary antibodies (Ab) were purchased from the commercial sources: a rabbit anti-α7 nAChR Ab from Genescript (Piscataway, NJ); Anti-NF-κB/p65 mAb from Cell Signal Technology (Cell Signal Technology, #3033); an antibody against dimethyl-histone H3 (Lys9) from Millipore (Millipore, cat #.07–212), an anti-mouse CD146 Ab FITC-conjugated and a mouse anti-neuron (NeuN) Ab from eBiosciences (San Diego, CA); a mouse anti-CD44 Ab (sc-7297); a rabbit anti-CD54 Ab (ICAM-1, 250593) from Abbiotec (San Diego, CA); a rabbit anti-β-actin (sc-7210) and an anti-GFAP Ab from Santa Cruz Biotechnology (Santa Cruz, CA); an anti-mouse CD146 Ab FITC-conjugated from Biolegend (San Diego, CA), and a rabbit anti-S100B Ab from BD Biosciences.

Evans blue (EB), L-(−)-Fucose, biotinylation kit and Caveolae/Rafts Isolation kit were purchased from Sigma-Aldrich (St. Louis, MO). Dynabeads M-450 Tosylactivated was purchased from Invitrogen (Carlsbad, CA). Ulex europaeus I (UEA I) lectin and mounting medium with DAPI were purchased from Vector (Buringame, CA). The HIV-1 gp41-I90 ectodomain peptide (gp41-I90) was prepared as previously described [37 (link)]. Recombinant HIV-1 Tat clade B protein and HIV-1 p24 recombinant were purchased from Prospec (Rehovort, Israel). All primary antibodies (Ab) were purchased from the commercial sources: a rabbit anti-MSFD2 Ab (sc-135305), a rabbit anti-CD44 Ab, a rat anti-Ly6C Ab and a rabbit anti-ICAM-1 Ab (Abcam, USA), a PE-conjugated anti-CD146 Ab (12-1469-41) and a PE-conjugated rat anti-mouse Ly6C Ab from eBiosciences (San Diego, CA, USA). The rest chemicals were obtained from Ding Guo Chang Sheng Company, Beijing, China.

Nicotine tartrate (NT), n-methyl-D-aspartate (NMDA), Ampicillin (Amp), kynurenic acid (Kyn) and dextromethorphan (DM) were purchased from Sigma-Aldrich (St. Louis, MO). Memantine (hydrochloride) was purchased from Cayman(Ann Arbor, MI). Dynabeads M-450 Tosylactivated was purchased from Invitrogen (Carlsbad, CA). Ulex europaeus I (UEA I) lectin and mounting medium with DAPI were purchased from Vector (Buringame, CA). All primary antibodies (Ab) were purchased from the commercial sources: a rabbit anti-MSFD2 Ab (sc-135305), a goat anti-MMP-9 Ab (sc-6841) and a rabbit anti-NF-κB p65 (sc-109) from Santa CruzBiotechnology (Santa Cruz, CA); an anti- CD146 Ab PE-conjugated (12-1469-41) from eBiosciences, (San Diego, CA). Transwell filters (3 μm pore size, 6.5 mm diameter) was purchased from BD Biosciences (San Jose, CA).