Protein concentrations of atrial myocardial tissues were determined by the Bradford method (Bio-Rad) according to the supplier’s instructions. The HeLa cell lysate (Santa Cruz, Texas, USA) served as positive control. 20 μg protein extracts were electrophoresed on a 12 % acrylamide SDS-PAGE gel and immunoblotted onto PVDF membranes. The membranes were blocked for 1 h in PBST containing 5 % w/v nonfat dry milk. The primary antibodies, including anti-ACADM and ECH1 (Abcam, Cambridge, USA), were used to react with the blots at room temperature for 2 h. Immunoreactivity was revealed with horseradish peroxidase-conjugated secondary antibody. All specific values of proteins evaluated were standardized to GAPDH (GeneTex, CA, USA).
Anti acadm
Manufactured by Abcam
Sourced in United States, United Kingdom
Anti-ACADM is a primary antibody that recognizes the ACADM (medium-chain acyl-CoA dehydrogenase) protein. ACADM is an enzyme involved in the mitochondrial beta-oxidation of medium-chain fatty acids. This antibody can be used to detect and study the ACADM protein in various research applications.
Automatically generated - may contain errors
2 protocols using anti acadm
1
Quantifying Myocardial Protein Levels
2
Immunohistochemical Staining of Fatty Acid Metabolism Proteins
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IHC staining was conducted according to the manufacturer's instructions using the Histostain™ Plus Kit (Cat. No. SP-9001; ZSGB-BIO, Beijing, China). The primary antibodies used in this study were anti-PPARα (1:200, Cat. No. ab233078; Abcam, Cambridge, UK), anti-ACADM (1:200, Cat. No. 55210-1-AP; Proteintech, China), anti-ACAA1(1:50, Cat. No. 12319-2-AP; Proteintech, China), anti-ACSL1(1:200, Cat. No. 13989-1-AP; Proteintech, China), anti-ACSL4 (1:50, Cat. No. 22401-1-AP; Proteintech, China) and anti-ACSL5(1:100, Cat. No. A1270, Abclonal, China). Positive signals for PPARα-, ACADM-, ACAA1-, ACSL1-, ACSL4-, and ACSL5-were observed as granular yellow or brown masses in hepatocytes.
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