Each sample was subjected to Sigma GenElute Bacterial Genomic DNA extraction kit using the lysozyme utilizing Gram-positive bacterial preparation method to ensure lysis of both Gram-positive and Gram-negative bacterial cells. The protocol was followed according to the manufacturer’s instructions and DNA was resuspended in 200 μl sterile DNA-free PCR grade water. Sample genomic DNA concentration and purity were determined as above.
Sigma genelute bacterial genomic dna extraction kit
Manufactured by Merck Group
The Sigma GenElute Bacterial Genomic DNA Extraction Kit is a product designed for the rapid and efficient isolation of genomic DNA from a variety of bacterial species. The kit utilizes a silica-based membrane technology to capture and purify the DNA, which can then be used in various downstream applications such as PCR, sequencing, and other molecular biology techniques.
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2 protocols using sigma genelute bacterial genomic dna extraction kit
1
Comprehensive Bacterial DNA Extraction
2
Gram-positive and Gram-negative DNA Extraction
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Each mixed culture sample was subjected to Sigma GenElute Bacterial Genomic DNA extraction kit using the lysozyme utilizing Gram-positive bacterial preparation method to ensure lysis of both Gram-positive and Gram-negative cells, according to the manufacturer’s instructions. DNA purity and concentrations were established by NanoDrop spectrophotometry (Thermo Fisher Scientific, Wilmington, DE, United States), and a Qubit 2.0 Fluorimeter using the dsDNA HS assay kit (Thermo Fisher Scientific), respectively.
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