Virus-inoculated AC or N. benthamiana was routinely examined under long-wave length ultraviolet light (Upland UVP Model B 100AP) to check transient GFP expression and systemic spread of the recombinant viruses. Photographs were taken with a Zeiss Axiophot microscope with filters (excitation at 450–490 nm and long-pass emission at 520 nm or excitation at 546 nm and long-pass emission at 590 nm) through a Nikon Coolpix 995 digital camera (Li et al., 2011 (link)). Confocal imaging of the leaves was performed with a Zeiss LSM 710 three-channel microscope with an excitation light of 405 nm, and the emission was captured at 454–581 nm.
Lsm 710 three channel microscope
Manufactured by Zeiss
The LSM 710 is a three-channel confocal microscope system designed for advanced imaging applications. It features high-resolution imaging, flexible configuration options, and multitrack imaging capabilities.
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Lab products found in correlation
2 protocols using lsm 710 three channel microscope
1
Visualizing Recombinant Virus Spread
2
CFP-NbATG8f Autophagy Imaging
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Confocal imaging was performed as described (Han et al., 2015 (link)). The leaves were agroinfiltrated with autophagy marker CFP-NbATG8f for 60 hr expression, followed by an additional infiltration with 20 uM E-64d for 8 hr before being monitored by a Zeiss LSM 710 three-channel microscope with an excitation light of 405 nm, and the emission was captured at 454 to 581 nm. TEM observation was performed as described (Wang et al., 2013 (link)).
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