Total cell protein was extracted and western blotting was carried out as previously described.14 The primary antibodies anti‐SP1 (ab13370) and GAPDH (ab37168) were obtained from Abcam (Cambridge, UK). HRP‐conjugated goat anti‐rabbit IgG secondary antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and Pierce ECL Western Blotting Substrate was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Gapdh ab37168
Manufactured by Abcam
Sourced in United Kingdom, United States
GAPDH (ab37168) is a recombinant protein that corresponds to a partial sequence of the Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) protein. GAPDH is an enzyme involved in the glycolytic pathway, catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated goat anti rabbit igg secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Anti sp1 ab13370, by Abcam (1 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Sod assay kit, by Nanjing Jiancheng (1 mentions)
Chop 2895, by Cell Signaling Technology (1 mentions)
2 protocols using gapdh ab37168
1
Western Blot Analysis of SP1 Protein
2
Evaluation of TBPH and CUR in PSD-95 and BDNF Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TBPH (CAS: 26040-51-7) and CUR (CAS: 458-37-7) were purchased from AccuStandard ® Inc (New Haven, USA) and Sigma-Aldrich (St Louis, MO, USA), respectively. A mouse ELISA kit for PSD-95 was obtained from Enzyme-linked Biotechnology Co., Ltd (Shanghai, China). ELISA kits for BDNF and p-CREB were purchased from BlueGene (Shanghai, China). MDA and GSH colorimetric kits as well as SOD assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The rabbit antimouse GRP78 (ab108613) and GAPDH (ab37168) antibodies were purchased from Abcam (Cambridge, MA, USA). The rabbit anti-mouse PERK (#3192) and CHOP (#2895) antibodies were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). HRP-goat anti-rabbit (AS1107) and HRP-goat anti-mouse (AS1106) were purchased from Bioss (Beijing, China). All other chemicals used in the study were of the highest grade available commercially, unless specifically noted.
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