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Collagenase p

Manufactured by Boehringer Ingelheim
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Sourced in Germany

Collagenase-P is an enzyme preparation used for the dissociation and isolation of cells from various tissue types. It contains a mixture of enzymes, including collagenases, that are effective in breaking down the extracellular matrix, allowing for the release of individual cells from the surrounding connective tissue.

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Lab products found in correlation

Attune nxt, by Thermo Fisher Scientific (2 mentions) Apc rat anti mouse cd11b, by BD (1 mentions) Hank s solution, by Beyotime (1 mentions) Bv421 rat anti mouse f4 80, by BD (1 mentions) Pe hamster anti mouse cd11c, by BD (1 mentions) 12 well plate, by Corning (1 mentions) Il 1β, by R&D Systems (1 mentions)

Close spelling variants of this product

Collagenase

2 protocols using collagenase p

1

Tissue Digestion and FACS Immunophenotyping

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Freshly harvested pancreatic and colonic tissues were digested in 1.0 mg/mL collagenase-P (Boehringer, Mannheim, Germany) solution at 37°C for 15 min and filtered through 75 µm filters with hank’s solution (Beyotime, Shanghai, China). Single-cell suspensions were incubated for 30 min at 4°C in hank’s solution with the following mAbs: APC Rat Anti-Mouse CD11b, BV421 Rat Anti-Mouse F4/80, Alexa Fluor 700 Rat Anti-Mouse Ly-6G, PE Hamster Anti-Mouse CD11c, FITC Rat Anti-Mouse MHCII, and PerCP-Cy5.5 Rat Anti-Mouse CD8a (BD Pharmingen, CA, USA). Gating method of fluorescence-activated cell sorting was programmed as CD11b+ Ly-6G+ (for neutrophils), CD11b+ F4/80+ (for macrophages), CD11c+ MHCII+ (for conventional dendritic cells, cDCs), and CD8a+CD11c+ MHCII+ (for plasmacytoid dendritic cells, pDCs). Flow cytometer was performed on Attune NxT (Thermo Fisher Scientific, MA, USA). Data were analyzed using ACEA NovoExpress software (Novo Express International, Inc., South San Francisco, CA, USA).
He Y., Wu C., Li J., Li H., Sun Z., Zhang H., de Vos P., Pan L.L, & Sun J. (2017). Inulin-Type Fructans Modulates Pancreatic–Gut Innate Immune Responses and Gut Barrier Integrity during Experimental Acute Pancreatitis in a Chain Length-Dependent Manner. Frontiers in Immunology, 8, 1209.
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2

Cytokine Expression in IVD Cells

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IVDs were obtained from human spine segments (donor age range 21–75 years) procured by the Gift of Hope Human Donor and Tissue Network of Illinois (approved by the Institutional Review Board; ORA #L01012604). IVDs were dissected within 24 hours from time of death, immediately after the spine segments were transported to the laboratory. AF and NP were separated. Cells were released by 0.4% Pronase (Calbiochem/Millipore) for 1 hour followed by 0.025% collagenase-P (Boehringer Ingelheim) digestion overnight. Cells were then plated in monolayer at 4×104 cells/cm2 in 12-well plates (Corning Life Sciences) with 1ml/well of DMEM/F12 medium with 20% FBS, until confluent. Cells were then serum-starved for 24 hours, and then stimulated with IL-1β (10 ng/mL; R&D systems, MN) in serum-free media for 24 hours. IL-8, IL-7 and IL-10 gene expression was analyzed using real-time PCR. IL-8 protein in the conditioned media was quantified using ELISA (Invitrogen, CA).
Zhang Y., Chee A., Shi P., Adams S.L., Markova D.Z., Anderson D.G., Smith H.E., Deng Y., Plastaras C.T, & An H.S. (2016). Intervertebral Disc Cells Produce Interleukins Found in Patients with Back Pain. American journal of physical medicine & rehabilitation / Association of Academic Physiatrists, 95(6), 407-415.
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