El6000 metal halide lamp

Manufactured by Leica

The EL6000 is a metal halide lamp manufactured by Leica. It is a high-intensity light source designed for microscopy and other laboratory applications. The lamp produces a bright, uniform illumination field suitable for a variety of imaging and analysis tasks. Its core function is to provide stable, consistent, and efficient illumination for various microscopy techniques.

Automatically generated - may contain errors

Lab products found in correlation

Dm6000 microscope, by Leica (2 mentions) Dfc350 fx monochrome ccd camera, by Leica (2 mentions)

Spelling variants of this product

EL6000 (93 citations) EL6000 lamp (4 citations)

2 protocols using el6000 metal halide lamp

Widefield Fluorescence Microscopy of Exponentially Growing Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For widefield fluorescence microscopy exponentially growing cells were immobilized on an agarose pad (1% agarose in phosphate-buffered saline) and covered with a #1.5 coverslip. Images were acquired with a Leica DM6000 microscope equipped with a Leica EL6000 metal halide lamp and a Leica DFC350 FX monochrome CCD camera. Phase contrast imaging was performed with an HCX PLAPO 100x/1.40 NA objective, whereas differential interference contrast images were acquired with an HCX PL APO 100x/1.46 NA objective. Narrow band-pass filter sets (CFP: Ex BP436/20, Em BP480/40, GFP: Ex BP470/40, Em BP525/50, YFP: Ex BP510/20, Em BP560/40, Cy3: Ex BP545/30, Em BP610/75) were used for fluorescence imaging.
During image acquisition, saturated pixels were avoided. The raw images were saved for further image processing (see below and Supplementary materials).

Helgesen E., Fossum-Raunehaug S., Sætre F., Schink K.O, & Skarstad K. (2015). Dynamic Escherichia coli SeqA complexes organize the newly replicated DNA at a considerable distance from the replisome. Nucleic Acids Research, 43(5), 2730-2743.

+ Open protocol

+ Expand

Fluorescence Microscopy of Growing Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For fluorescence microscopy exponentially growing cells were immobilized on an agarose pad (1% agarose in phosphate-buffered saline) and covered with a #1.5 coverslip. Images were acquired with a Leica DM6000 microscope equipped with a Leica EL6000 metal halide lamp and a Leica DFC350 FX monochrome CCD camera. Phase contrast imaging was performed with an HCX PLAPO 100×/1.40 NA objective. Narrow band-pass filter sets (CFP: Ex BP436/20, Em BP480/40, YFP: Ex BP510/20, Em BP560/40, Cy3: Ex BP545/30, Em BP610/75) were used for fluorescence imaging.
During image acquisition, saturated pixels were avoided. The raw images were saved for further image processing (see below).

Helgesen E., Sætre F, & Skarstad K. (2021). Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli. Scientific Reports, 11, 474.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!