Diaminobenzadine

Manufactured by Agilent Technologies

Sourced in United States

Diaminobenzadine is a chemical compound used as a sensitive substrate for the detection of peroxidase enzymes in various laboratory applications. It is a commonly used reagent in immunohistochemistry, immunocytochemistry, and other biochemical assays.

Automatically generated - may contain errors

Lab products found in correlation

Peroxidase block, by Agilent Technologies (2 mentions) Avidin block, by Vector Laboratories (2 mentions) Biotin block, by Vector Laboratories (2 mentions) Protein block serum free, by Agilent Technologies (1 mentions) Streptavidin hrp, by Biocare Medical (1 mentions) Goat anti rabbit biotinylated igg, by Biocare Medical (1 mentions)

2 protocols using diaminobenzadine

Immunohistochemical Analysis of Kidney Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Extracted kidneys were placed into a neutral buffered 10% formalin solution. The kidney sections were cut at 4 μm slices, dried, and deparaffinized for subsequent streptavidin-biotin immunohistochemistry. After deparaffinization, the slides were treated with a citrate buffer pH6 for a total of 35 min. The slides were blocked with a peroxidase block (DAKO, Carpinteria, California, USA), Avidin Block (Vector Labs, Burlingame, California, USA), Biotin Block (Vector Labs), and serum-free Protein Block (DAKO). For morphological analysis, the tissue was stained with hematoxylin-eosin. For immunohistochemical staining, tissue sections were incubated for 30 min with anti-α, β or γ-ENaC antibodies. Secondary detection was performed with goat anti-rabbit biotinylated immunoglobulin G (IgG) (Biocare, Concord, California, USA) followed by streptavidin conjugated with horseradish peroxidase (Biocare) and visualized with diaminobenzadine (DAKO).

Pavlov T.S., Levchenko V., Ilatovskaya D.V., Moreno C, & Staruschenko A. (2016). Renal sodium transport in renin-deficient Dahl salt-sensitive rats. Journal of the Renin-Angiotensin-Aldosterone System: JRAAS, 17(3), 1470320316653858.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Renal ENaC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Extracted kidneys were placed into a neutral buffered 10% Formalin solution. The kidney sections were cut at 4 μm slices, dried and deparaffinized for subsequent streptavidin-biotin immunohistochemistry. After deparaffinization, the slides were treated with a citrate buffer pH6 for total of 35 min. The slides were blocked with a peroxidase block (DAKO, Carpinteria, CA, USA), Avidin Block (Vector Labs, Burlingame, CA, USA), Biotin Block (Vector Labs), and serum-free Protein Block (DAKO). For morphological analysis, the tissue was stained with haematoxylin-eosin. For immunohistochemical staining, tissue sections were incubated for 30 min with anti-α, β or γ-ENaC antibodies. Secondary detection was performed with Goat anti-Rabbit biotinylated IgG (Biocare, Concord, CA, USA) followed by Streptavidin HRP (Biocare) and visualized with Diaminobenzadine (DAKO).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!