H10010

Six-week-old C57BL/6J mice were housed in a specified pathogen-free animal room with a 12-h light/12-h dark cycle and free access to food and water. For diet-induced NAFLD, 6-week-old male C57BL/6J mice were randomized into two groups: one group was fed a HFD (60.9% fat, 21.8% carbohydrate, and 18.3% protein; H10060) for 16 weeks, and the other group was fed a normal chow diet (NCD) (4% fat, 78% carbohydrate, and 18% protein; H10010, HFK Bioscience, Beijing, China). Gene-edited ob/ob mice provided by HFK Bioscience (Beijing, China) were fed an NCD for 8 weeks. The mice were sacrificed at the time point, blood samples were collected for testing, and liver tissue was collected for fixation with paraformaldehyde or storage with frozen liquid nitrogen. All experimental procedures in this study were approved by the Animal Use Subcommittee of Tongji Medical College of Huazhong University of Science and Technology.

B6.129S (FVB)-Bcl6tm1.1Dent/J(Bcl6fl/fl) were obtained from the Jackson Laboratory (Stock No.: 023727). Alb-Cre mice were obtained from the Model Animal Research Center of Nanjing University. Alb-Cre mice were bred with the Bcl6 fl/fl mice to obtain the Alb-Cre Bcl6Δ mice. Genotyping was performed via PCR using previously published protocols [28 (link)–30 (link)]. The Animal Use Subcommittee of Tongji Medical College of Huazhong University of Science and Technology approved all animal experimental procedures. A specific-pathogen-free facility equipped 12-h light/12-h dark cycles and free access to food and water were used to house six-week-old C57BL/6J mice.
Six-week-old male mice were randomized into two groups to create diet-induced NAFLD – the first of these groups were fed 16 weeks of normal chow diet (NCD) (4% fat, 78% carbohydrate, and 18% protein; H10010, HFK Bioscience, Beijing, China) while the other group received 16 weeks of HFD (60.9% fat, 21.8% carbohydrate, and 18.3% protein; H10060). NCDGene-edited ob/ob mice provided by HFK Bioscience (Beijing, China) were fed NCD for 8 weeks. After this duration, mice were sacrificed, blood samples were harvested, and livers were dissected for immediate paraformaldehyde fixation or storage for further use with frozen liquid nitrogen.

Lewis homozygous (dw/dw) rats were provided by Professor Michael J Waters, University of Queensland, Australia. Lewis wild-type (WT) rats were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). Lewis heterozygote (dw/+) rats were generated by mating Lewis dw/dw and Lewis WT rats. Lewis dw/dw rats at 37 weeks (five females and five males), and Lewis dw/+ rats at 37 weeks (five females and five males) were analyzed in our study. The rats were housed in a standard 12h light/dark cycle environment with sufficient water and food (SF; 10% kcal fat, H10010, Beijing HFK Bioscience Co., Ltd., Beijing, China). The rats were in narcotism by injecting 2.5% tribromoethanol into the cavum abdominis (15 mL/kg). All rats were weighed before sacrifice. The body lengths were measured from the nose’s tip to the tail’s tip after sacrifice. The intact livers were collected and then weighed. The ethics committee of Peking Union Medical College Hospital approved the animal experiment protocols (XHDW-2021-035).