The 727-compound NCC was purchased from Evotec (Hamburg, Germany) as 10-mM solutions in DMSO. The pig skeletal muscle (longissimus dorsi) was processed and purchased from Pel-Freez Biologicals (AR, USA). Fluorophores Alexa Fluor 488 C5-maleimide (AF488) and Alexa Fluor 568 C5-maleimide (AF568) were purchased from Life Technologies (OR, USA). Individual NCC compounds of interest (hits) were purchased separately, as following: disulfiram and ebselen from Fisher Scientific; chloroxine, diazoxide and fluphenazine from Sigma-Aldrich; cefatrizine propylene glycol (PG), and cefixime trihydrate and tacrolimus from Sequoia Research Products Ltd (Pangbourne, UK); and pravastatin and lofepramine from Santa Cruz Biotechnology (CA, USA).
Disulfiram
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States
Disulfiram is a laboratory chemical used as a reagent in various analytical and research applications. It is a crystalline solid and a member of the thiuram compounds class. Disulfiram's primary function is to serve as a chemical agent in specific experimental procedures and analytical techniques, but a detailed description of its intended use is not available.
Automatically generated - may contain errors
2 protocols using disulfiram
1
High-throughput Screening of Compound Library
2
Novel Chemical Compounds Screening
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Disulfiram was purchased from Fisher Scientific (Hampton, NH, USA). RI171 and RI172 were purchased from MolPort (Rīga, Latvia) while RI173 was purchased from Ambeed (Arlington Heights, IL, USA). RI174, RI175, RI176, and RI177 were obtained from Sigma-Aldrich (St. Louis, MO, USA). All compounds were dissolved in dimethyl sulfoxide. All solvents were reagent grade, and all reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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