Potassium acetate

2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ibuprofen, lidocaine hydrochloride, and caffeine were purchased from Sigma Aldrich (Poznań, Poland); disodium phosphate and potassium dihydrogen phosphate from Merck, Darmstadt (Germany); Biobase, beeswax were purchased from Mazidła.com (Poznań, Poland); grape seed oil was purchased from Monini (Spoleto, Italy); sodium acetate anhydrous, potassium persulfate, potassium acetate, xylene, 99.5% acetic acid, aluminium chloride, 36% hydrochloric acid, sodium chloride, potassium chloride, sodium sulphate anhydrous as well as propylene glycol, ethanol, and methanol were from Chempur (Piekary Śląskie, Poland), whereas acetonitrile for HPLC from J.T. Baker, (Landsmeer, The Netherlands). All reagents were of analytical grade.

2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2carboxylic acid (Trolox), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), 3,4-dihydroxybenzoic acid, chlorogenic acid, caffeic acid, acetylsalicylic acid, bovine albumin serum (BSA), linoleic acid sodium salt (≥98%), quercetin, rutin, and soybean lipoxygenase were purchased from Sigma Aldrich (Steinheim am Albuch, Germany); Folin–Ciocalteu reagent, gallic acid, 4-hydroxybenzoic acid, 3-hydroxybenzoic acid, disodium phosphate, propylene glycol, and potassium dihydrogen phosphate from Merck, Darmstadt (Germany); sodium acetate anhydrous, potassium persulfate, potassium acetate, 99.5% acetic acid, 36% hydrochloric acid, sodium chloride, potassium chloride, ethanol, methanol, acetone, and phosphate-buffered saline (PBS; pH 7.00 ± 0.05) were from Chempur (Piekary Śląskie, Poland), whereas acetonitrile for HPLC was from J.T. Baker (Berlin, Germany). Hydroxyethylcellulose, 1,10-phenanthroline, and succinyl-alanyl-alanyl-prolyl-valine chloromethylketone (SPCK, Abcam, Cambridge, MA, USA) were used as received. All reagents were of analytical grade.

The chemicals 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ) were purchased from Sigma Aldrich (Poland). Folin–Ciocalteu reagent was supplied by Merck (Darmstadt, Germany). Anhydrous sodium acetate, potassium persulfate, potassium acetate, ferric chloride, 36% hydrochloric acid, and ethyl alcohol were obtained from Chempur (Piekary Śląskie, Poland). All reagents were of analytical grade.