Anti cd45ro

Immunophenotyping was performed by flow cytometric analysis (five-colour flow cytometer FC-500; Beckman Coulter with CXP software).
For the staining of T-lymphocyte differentiation stages the following antibodies were used: R-Phycoerythrin-Cyanine 7 (PC7)-conjugated anti-CD4, anti-CD8, Phycoerythrin-Texas red X (ECD)-conjugated anti-CD8, anti-CD45RO; PC5-conjugated anti-CD27, and anti-CD25 (all from Beckman Coulter). For the staining of T_regs we used anti-CD4 PC7, anti-CD127 PE, and anti-CD25 PC5 (Beckman Coulter).
T lymphocytes, both CD4 and CD8, were divided into naïve CD45RO^-CD27⁺, early differentiated (ED) CD45RO⁺CD27⁺, late differentiated (LD) CD45RO⁺CD27^-, and fully differentiated effector (FD) CD45RO^-CD27^- memory T cells. In CD8⁺ cells a unique CD45RO^-CD27^dim intermediate population was determined as well [22 (link)] (Fig. 1). T_reg cells were defined as CD4⁺CD25^highCD127^low [24 (link)] in accordance with recent studies that demonstrated that the description of T_regs as a CD4⁺CD25^highCD127^low phenotype correlates well with FoxP3 expression [25 (link), 26 (link)].