All reagents were used as received without further purification. Polyvinylpyrrolidone (PVP, Mw = 1.3 × 106), N,N-dimethylformamide (DMF), Cu(Ac)2·H2O, CH3COOH, CH3COONa, guaiacol, vanillin, phenol and 3,5-dinitrosalicylic acid were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Polyacrylonitrile (PAN, Mw = 50,000–60,000) was purchased from Zhejiang Shangyu Wuyue Trade Co., Ltd. (Shangyu, Chaina). Catechol was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Laccase (Lac) and Nafion were purchased from Sigma-Aldrich Chemical Co., Ltd. (St. Louis, MO, USA). All water was deionized. A 0.1 M acetate buffer solution was employed as supporting electrolyte.
Guaiacol
Manufactured by Sinopharm
Sourced in China
Guaiacol is a colorless, aromatic organic compound commonly used as a laboratory reagent. It is a phenolic compound derived from guaiacum, a tree native to the Caribbean. Guaiacol serves as a precursor for various chemical synthesis reactions and is used for analytical purposes in research and development settings.
Automatically generated - may contain errors
Lab products found in correlation
Phenol, by Sinopharm (4 mentions)
Sodium hydroxide (naoh), by Tianjin Damao (3 mentions)
Vanillin, by Sinopharm (2 mentions)
Catechol, by Sinopharm (2 mentions)
3 5 dinitrosalicylic acid, by Sinopharm (1 mentions)
Ch3coona, by Sinopharm (1 mentions)
Ch3cooh, by Sinopharm (1 mentions)
Laccase lac, by Merck Group (1 mentions)
Nafion, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Sinopharm (1 mentions)
Polyvinylpyrrolidone, by Sinopharm (1 mentions)
Furfural, by Sinopharm (1 mentions)
Acetonitrile of hplc grade, by Merck Group (1 mentions)
2 4 6 tri 2 pyridyl 1 3 5 triazine, by Merck Group (1 mentions)
Folin ciocalteu, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Hydrogen peroxide solution, by Sinopharm (1 mentions)
Sulphuric acid, by Sinopharm (1 mentions)
Foline phenol, by Yuanye Bio-Technology (1 mentions)
8 protocols using guaiacol
1
Enzymatic Oxidation of Phenolic Compounds
2
Saccharomyces cerevisiae Ethanol Fermentation
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The microorganism used for fermentationwas Saccharomyces cerevisiae in the form of dry yeast (thermal resistant) (Angel Yeast Company Ltd, Yichang, China), which was named Angel Super-Alcohol Active Dry Yeast (starch base). The yeast was kept at 4 oC during storage, and was weighted and directly added to specified fermentation media as received right before each use. Filter paper activity of cellulase (CTec-2) was 150 FPU/mL, kindly provided by Novozymes Investment Co. Ltd. (Beijing, China). D-glucose (C6H12O6·H2O), glycerol (99 wt%), ethanol (99 wt%) were received from Sinopharm (China). Phenol, guaiacol, vanillin, levulinic acid, furfural and 5-hydrolsymethylfurfural (HMF), were received from Sinopharm (China). Polyethylene glycol were purchased from Aladdin(China). Sulphuric acid (98wt%) and methylene blue were provided by a local supplier. Deionized water (DI H2O) was produced by a Milli-Q Integral 5 system. All other chemicals were of analytical quality.
3
Phenolic Compounds in Solid Fuels
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Gas-phase model compounds, Phenol, guaiacol and syringol, were used as Phenolic representatives of structural entities in solid fuels and tar. Phenol, guaiacol and syringol were purchased from Sinopharm Chemical Reagent Co., Ltd, China. All samples were of analytical grade and used without any further purification.
4
Synthesis of Epoxy Resin Hardener
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Guaiacol was purchased from Sinopharm Chemical Reagent Co., Ltd, China. Succinic anhydride and benzyltriethylammonium chloride were purchased from Saen Chemical Technology Co., Ltd., Shanghai, China. 4,4ʹ-methylenedianiline was obtained from Mackliin Biochemical Co., Ltd., Shanghai, China. Epichlorohydrin was supplied by Aladdin-reagent Co., Ltd., Shanghai, China. Aluminum chloride anhydrous, hydrazine hydrate, 1,2-dichloroethane, sodium hydroxide, and sodium sulfate anhydrous were purchased from Damao Chemical Reagent Factory, Tianjin, China. Epoxy resin (DGEBA, trade name E51, epoxy value 0.51 mol·100 g−1) was purchased from Nantong Xingchen Synthetic Materials Co., Ltd, China. All chemicals and solvents were used without further purification.
5
Zeolite Catalysts Synthesis and Characterization
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The following materials were provided by various suppliers: guaiacol (Sinopharm Chemical Reagent Co., Ltd., China), decalin (Tokyo Chemical Industry). Hβ with different Si/Al ratios and HUSY were purchased from Catalyst Factory of Nankai University (Tianjin, China). Hβ with a Si/Al ratio of X was denoted as Hβ(X). These purchased zeolites were calcined at 560 °C for 4 h prior to use.
6
Antioxidant Capacity Measurement Protocols
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Folin–Ciocalteu, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tri-2-pyridyl-1,3,5-triazine (TPTZ), 7,8-tetramethyl-chroman-2-carboxylicacid (Trolox), and ascorbic acid were purchased from Sigma Aldrich (St. Louis, MO, USA). Guaiacol was supplied by Sinopharm Chemical Reagent (Shanghai, China). Acetonitrile of HPLC grade was purchased from Merck(Darmstadt, Germany). N2 (99.999%) gases were purchased from Beijing Beiwen Gas Co., Ltd. (Beijing, China). Other analytical-grade chemicals were provided by Beijing Chemicals Co., Ltd. (Beijing, China).
7
Analytical Techniques for Biochemical Characterization
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The formic acid, acetonitrile, and methanol used for high-performance liquid chromatography (HPLC) analysis were chromatographic grade and purchased from Merck KGaA (Shanghai, China). The ethanol, anthrone, sulphuric acid, glucose, DNS (3,5dinitrosalicylic acid), phenol, hydrochloric acid, sodium carbonate, sodium dihydrogen phosphate, disodium hydrogen phosphate, catechol, guaiacol, and hydrogen peroxide solution were all analytical purity (AR) and obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The bovine serum albumin (BSA), Coomassie Bright Blue G-250, Foline-phenol, and gallic acid were purchased from Yuan-ye Bio-Technology Co., Ltd. (Shanghai, China).
The ultraviolet spectrophotometer (UV-1800) was purchased from Shimadzu Co., Ltd. (Kyoto, Japan). The analytical balance (XS 250) was obtained from METTLER TOLEDO (Columbus, OH, USA). The high-speed centrifuge (2-16R) was purchased from HENGNUO Instrument Equipment Co., Ltd. (Changsha, Hunan, China).
The ultraviolet spectrophotometer (UV-1800) was purchased from Shimadzu Co., Ltd. (Kyoto, Japan). The analytical balance (XS 250) was obtained from METTLER TOLEDO (Columbus, OH, USA). The high-speed centrifuge (2-16R) was purchased from HENGNUO Instrument Equipment Co., Ltd. (Changsha, Hunan, China).
8
Papaya Fruit Oxidative Stress Induction
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Papaya (Carica papaya L. cv. Huakangyihao) fruit were harvested at a mature stage from a commercial orchard in Guangzhou, China, and then selected for uniformity of shape, color, and size. Blemished or diseased fruits were discarded during sampling.
N,N-dimethyl-4,4 -bipyridinium dichloride (Paraquat) and FeSO 4 /H 2 O 2 (1:1) were used to produce O 2-and •OH as described by Takizawa [41] and Halliwell [42], respectively. Paraquat, catechol, and guaiacol were obtained from Sinopharm Chemical Reagent Co., Guangzhou, China.
N,N-dimethyl-4,4 -bipyridinium dichloride (Paraquat) and FeSO 4 /H 2 O 2 (1:1) were used to produce O 2-and •OH as described by Takizawa [41] and Halliwell [42], respectively. Paraquat, catechol, and guaiacol were obtained from Sinopharm Chemical Reagent Co., Guangzhou, China.
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