Body composition analyzer ecomri

Six-week-old animals (control, Prdm16 Tg, Ucp1−/−, and Prdm16 Tg x Ucp1−/− mice) were fed a HFD (D12492, Research Diet) or a regular diet (RD) under the ambient temperature at 22°C or thermoneutrality at 30°C for 12–24 weeks. HFD feedings were started at 6 weeks old. Body weight was measured every week. Whole-body energy expenditure (VO₂, VCO₂), food intake, and locomotor activity (beam break counts) were monitored using a Comprehensive Lab Animal Monitoring System (CLAMS, Columbus Instruments). CLAMS experiment in response to cold exposure was performed in mice at 10–12 weeks old under RD. Locomotor activity was measured by CLAMS for 5 days in mice at 12 weeks of HFD. Fat mass and lean mass were measured by the Body Composition Analyzer EcoMRI (Echo Medical Systems). For GTT experiments, mice were fed HFD or RD for 10 weeks. After an overnight fast, the mice were injected intraperitoneally with glucose (1.5 g/kg). For ITT experiments, the mice on HFD or for 11 weeks were injected intraperitoneally with insulin (0.75 U/kg for mice under HFD and 0.2U/kg for mice under RD) after 3 hours of fasting. Blood samples were collected at indicated time points, and glucose levels were measured using blood glucose test strips (Abbott).