The 4% paraformaldehyde-fixed and paraffin-embedded longissimus dorsi tissue sections
were dewaxed in xylene, followed by rehydration in decreasing ethanol concentrations.
After antigen retrieval by high-temperature heating in citrate buffer (pH=6.0) and
blocking in inhibitor buffer (3% H2O2), the slides were incubated
with anti-glucose transporter GLUT4 primary antibody (Abcam, Cambridge, UK , Cat. ab654)
overnight at 4°C. Signals were visualized by 3,3′-diaminobenzidine (DAB); brown staining
represents GLUT4, and blue staining represents nuclei. Average optical density values of
every field were measured using Image-Pro Plus 6.0 software.
were dewaxed in xylene, followed by rehydration in decreasing ethanol concentrations.
After antigen retrieval by high-temperature heating in citrate buffer (pH=6.0) and
blocking in inhibitor buffer (3% H2O2), the slides were incubated
with anti-glucose transporter GLUT4 primary antibody (Abcam, Cambridge, UK , Cat. ab654)
overnight at 4°C. Signals were visualized by 3,3′-diaminobenzidine (DAB); brown staining
represents GLUT4, and blue staining represents nuclei. Average optical density values of
every field were measured using Image-Pro Plus 6.0 software.