Bombyx mori raw silk (20/22D, 6A) was purchased from HaiAn Tian Xin Silk Industry (Nantong, China), Cellulose membrane (14,000 Da, MD44) was purchased from Union Carbide (Danbury, CT, USA). The chemicals such as Sodium carbonate (Na2CO3, ≥99%), Calcium chloride (CaCl2, ≥98%), Dibasic sodium phosphate (Na2HPO4, ≥98%), and Coomassie Brilliant Blue (≥55%) Tris-(hydroxymethyl) aminomethane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Lithium bromide (LiBr, ≥99%) was purchased from Finecollection Institute of Chemical Industry (Hefei, China). Sodium dodecyl sulfate (SDS, ≥99%), Ammonium persulphate (≥99%), Acrylamide (≥99%), N,N,N′,N′-Tetramethylethylenediamine (≥99%), Tris Base were purchased from Sigma Aldrich (St. Louis, MO, USA).
Dibasic sodium phosphate na2hpo4
Manufactured by Sinopharm
Sourced in China
Dibasic sodium phosphate (Na2HPO4) is a chemical compound commonly used in laboratory settings. It is a white, crystalline solid that is soluble in water. Dibasic sodium phosphate serves as a buffering agent, helping to maintain a specific pH level in aqueous solutions.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium persulphate, by Merck Group (1 mentions)
N n n n tetramethylethylenediamine, by Merck Group (1 mentions)
Coomassie brilliant blue, by Sinopharm (1 mentions)
Tris hydroxymethyl aminomethane, by Sinopharm (1 mentions)
Tris base, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Sodium carbonate, by Sinopharm (1 mentions)
Calcium chloride, by Sinopharm (1 mentions)
Potassium chloride (kcl), by Sinopharm (1 mentions)
Phosphate buffer, by Merck Group (1 mentions)
Pdms sylgard 184, by Dow (1 mentions)
Cell counting kit cck 8, by AbMole (1 mentions)
Genipin, by J&K Scientific (1 mentions)
Streptozotocin (stz), by AbMole (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Acetic acid, by Sinopharm (1 mentions)
Bovine serum albumin 3 3 5 5 tetramethyl benzidine, by Merck Group (1 mentions)
Sulfuric acid h2so4, by Sinopharm (1 mentions)
96 well elisa plate, by Corning (1 mentions)
4 protocols using dibasic sodium phosphate na2hpo4
1
Silk Protein Extraction and Purification
2
Fabrication of Nanomaterial-based Composites
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Potassium chloride (KCl), sodium chloride (NaCl), sodium sulphate (Na2SO4), dibasic sodium phosphate (Na2HPO4), potassium dihydrogen phosphate (KH2PO4), ascorbic acid, and glucose were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Antibiotics including CAP, Erythromycin, Malachite green, Nitrofurazone, Metronidazole, and Ciprofloxacin were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd., Shanghai, China. All of the above chemical reagents were analytical reagents and were used without further purification. CNTs (diameter: 10–20 nm, length: 10–30 μm) ethanol dispersion and AgNWs (diameter ≈ 60 nm, length ≈ 10 μm) ethanol dispersion were purchased from XFNANO Materials Technology Co., Ltd., Nanjing, China, and Nanjing JCNANO Technology Co., Ltd., Nanjing, China, respectively. Deionized Milli-Q water (18.2 MΩ/cm) was used throughout the experiments.
3
Hydrogel-based Biomaterial Characterization
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PDMS (Sylgard 184) was obtained from Dow Corning (Midland, USA). PLA was purchased from Lakeshore Biomaterials Inc. (AL, USA). Cell counting kit (CCK-8) and STZ were purchased from AbMole BioScience (TX, USA). Gel (from cold water fish skin and from porcine skin), FITC, phosphate buffer, and deuterium oxide (D2O, D > 99.9%) were purchased from Sigma-Aldrich (MO, USA). Ehrlich’s reagent was purchased from BioTechnology Co Ltd. (Shanghai, China). Acetic acid, sodium carbonate anhydrous (Na2CO3), and dibasic sodium phosphate (Na2HPO4) were obtained from Sinopharm Chemical Reagent Co Ltd. (Beijing, China). Insulin (short-, intermediate-, and long-acting types) was purchased from Wanbang Biopharmaceuticals Co. Ltd. (Jiangsu, China). HA (Mw ≈ 350,000 Da) was purchased from Toronto Research Chemicals Inc. (Toronto, Canada). BDDE (Mw = 202.25 Da) was purchased from Adamas Reagent Co. Ltd. (Basel, Switzerland). Genipin was purchased from J&K Scientific (Beijing, China). Mouse fibroblast cell lines (L-929 cells) were purchased from iCell Bioscience (Shanghai, China), and female Balb/c mice were obtained from the Institute of Laboratory Animal Sciences (Beijing, China). Aqueous solutions were carried out using ultrapure water from a reverse osmosis membrane system, unless otherwise stated.
4
Quantification of Genetically Modified Proteins
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Streptavidin (SA), hemoglobin, horseradish peroxidase (HRP), bovine serum albumin (BSA), 3,3′,5,5′-tetramethylbenzidine (TMB) and polyvinyl pyrrolidone (PVP) were obtained from Sigma-Aldrich. 2-methylimidazole, zinc nitrate (Zn (NO3)2), tween-20, sulfuric acid (H2SO4), potassium dihydrogen phosphate (KH2PO4), dibasic sodium phosphate (Na2HPO4), potassium chloride (KCl) and sodium chloride (NaCl) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 96-well ELISA plates were bought from Corning-Costar. CP4-EPSPS, Cry1Ab, Cry1e, biotin-antibody and monoclonal antibody to Cry1Ab were acquired from Youlong Biotech Co., Ltd. (Shanghai, China). All chemical reagents were used as received without further purification.
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