Single cell suspensions were prepared for surface staining of the cells with fluorochrome-conjugated antibodies against Flt3 (A2F10), Bst2 (ebio927), and Siglec-H (ebio440c) were purchased from eBioscience, B220 (RA3-6B2), CD4 (RM4-5), CD8 (53-6.7), CD11b (M1/70), CD3 (145-2c11), and CD86 (GL1) were purchased from BD, CD69 (H1.2F3), CD19 (6D5), CD49b (Dx5), MHCII (M5/114.15.2), and Ly-6c (HK1.4) were purchased from BioLegend, and Ly49Q (2E6) were purchased from Medical & Biological Laboratories Co. After incubating on ice for 15 min, the cells were washed twice and analyzed by BD FACS canto II flow cytometer. In some experiments, mouse splenic pDCs and cDCs were sorted by BD FACS Aria II system.
Ebio927
Manufactured by Thermo Fisher Scientific
The ebio927 is a piece of laboratory equipment designed for general scientific applications. It provides core functionality for the intended use, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Cd86 gl1, by BD (1 mentions)
Ly6c hk1, by BioLegend (1 mentions)
Cd69 h1.2f3, by BD (1 mentions)
Ebio 440c, by Thermo Fisher Scientific (1 mentions)
Facsaria 2 system, by BD (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Ter119, by BioLegend (1 mentions)
Ter119, by BioXCell (1 mentions)
Mr9 4, by BioLegend (1 mentions)
Pc 61, by BioXCell (1 mentions)
M1 70, by BioXCell (1 mentions)
M1 70, by BioLegend (1 mentions)
Rb6 8c5, by BioXCell (1 mentions)
2 protocols using ebio927
1
Multi-Dimensional Immune Cell Profiling
2
Characterizing Mouse Immune Cell Phenotypes
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Antibodies directed against the following mouse markers were used: CD3 (145–2C11, Tonbo Biosciences 60–0031), CD4 (RM4–5, BioLegend 100559; GK1.5, BioXCell BE0003–1), CD8 (53–6.7, Tonbo Biosciences 75–0081; 53.6.72, BioXCell BE0004), CD11b (M1/70, BioLegend 101204; M1/70, BioXCell BE0007), CD11c (N418, BioLegend 117322), CD19 (6D5, BioLegend 115534), CD25 (PC61, BioLegend 102024; PC61.5.3, BioXCell BE0012), CD45 (30-F11, BioLegend 103138), CD45.1 (A20, BioLegend 110714), CD69 (H1.2F3, BioLegend 104504), CD80 (16–10A1, BioLegend 104724), B220 (RA3–6B2, BioLegend 103232; RA3.3A1/6.1, BioXCell BE0067), AIRE (5H12, eBioscience 53–5934–82), EpCAM (G8.8, BioLegend 118206), F4/80 (BM8, BioLegend 123116), Gr-1 (RB6–8C5, BioLegend 108410; RB6–8C5, BioXCell BE0075), I-A/I-E (M5/114.15.2, BioLegend 107628), NK1.1 (PK136, BioLegend 108716), PDCA1 (eBio927, eBioScience 12–3172–82), Sirpα (P84, BioLegend 144008), TER-119 (TER-119, BioLegend 116210; TER-119, BioXCell BE0183), Vα2 (B20.1, BioLegend 127818), Vβ5 (MR9–4, BioLegend 139504), and XCR1 (ZET, BioLegend 148212). For immunostaining ~107 cells in 100 μL of PBS + 2% bovine calf serum (BCS), fluorochrome-conjugated antibodies were diluted from stock concentrations of 0.5 mg mL−1 and incubated with cells for 20 min on ice, unless specified.
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