Nanoparticles mean hydrodynamic diameter was measured by dynamic light scattering (DLS) on a Malvern Zetasizer Nano ZS instrument. Both nanoparticles and nanocomposites were observed by high resolution transmission electron microscopy (HR-TEM) on a 200 kV Titan FEI HR-TEM. Thermal properties were characterized by modulated differential scanning calorimetry (MDSC) on a TA Q200 instrument. Nanotomography technique was carried out using Zeiss NVision 40 FIB/SEM dual beam instrument (400 slices of 3.5 nm). Thermal stability and particles weight or volume fraction were studied by TGA using a TA Instrument TGA Q500. Magnetic properties were measured by vibrating sample magnetometer (VSM) on a Microsense instrument and average film-conductivity was obtained with four-point method over 25 points on a TC1000 prober from Karl Suss. The complex permeability was measured from 10 MHz to 10 GHz by high performance coil-perturbation method using impedance analyzer (Agilent 4294A) combined with vectorial network analyzer (Agilent N5222A).
Nvision 40 fib sem
Manufactured by Zeiss
The NVision 40 FIB/SEM is a focused ion beam scanning electron microscope (FIB/SEM) system developed by Zeiss. It combines a high-performance field emission SEM and a gallium-based FIB column to provide advanced imaging and sample preparation capabilities. The system enables high-resolution imaging, site-specific sample modification, and precise milling for applications in materials science, nanotechnology, and other research fields.
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Lab products found in correlation
4 protocols using nvision 40 fib sem
1
Comprehensive Characterization of Nanoparticles and Nanocomposites
2
Hippocampal CA1 Stratum Radiatum Imaging
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We used the following procedure to mount specimens with minimal specimen charging. Regions of the hippocampus were first dissected under a stereoscopic microscope using a razor blade. Next, samples were mounted onto a flat, blank resin slab and trimmed with a glass knife using an ultramicrotome in preparation for the electron microscope. The block containing hippocampal CA1 stratum radiatum was imaged inside an NVision 40 FIB‐SEM (Carl Zeiss) using an acceleration voltage of 1.5 kV, a current of 350 pA, and a dwell time of 10 μs/pixel. With the repeated use of a FIB, directed parallel to a block face to remove (or mill) a thin layer of embedded tissue, serial images were collected at a magnification of 6 nm/pixel. The milling depth between images was 5 nm, resulting in perfectly isotropic voxels. Each exposed surface was imaged using backscattered electrons.
3
In Situ Solid-State Lithiation of Germanium
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The in situ experiment was performed on a Zeiss Nvision 40 FIB-SEM at the Center for Nanoscale Materials, Argonne National Laboratory. Figure S1 shows the schematic and SEM image of the experimental setup. Li metal was placed on the SEM stub as the negative electrode.
A µm-sized Ge particle was attached to a tungsten probe by FIB carbon deposition as the positive electrode. The Ge particles were prepared by jet milling as shown in our previous paper. 20 A small piece of solid electrolyte (LLZTO) was placed on the Li Li 6.4 La 3 Zr 1.4 Ta 0.6 O 12 metal. The preparation of LLTZO can be found in Ref. 21 . A Keithley 6430 sub-femtoamp remote sourcemeter was used to control the voltage to operate the single particle battery. During lithiation, the Ge particle was attached to the solid electrolyte and a bias voltage of -0.01 V was applied to the Ge particle. The all solid single particle battery provides the opportunity to observe the particle morphology change in operando. SEM images were taken every 5 s. To minimize the effect of the electron beam, we reduced the imaging current to about 40 pA, about 1% of the lithiation current. The TEM analysis was performed on a JEOL JEM2100F operated at 200 kV.
The TEM sample was prepared by a standard FIB lift-out procedure.
A µm-sized Ge particle was attached to a tungsten probe by FIB carbon deposition as the positive electrode. The Ge particles were prepared by jet milling as shown in our previous paper. 20 A small piece of solid electrolyte (LLZTO) was placed on the Li Li 6.4 La 3 Zr 1.4 Ta 0.6 O 12 metal. The preparation of LLTZO can be found in Ref. 21 . A Keithley 6430 sub-femtoamp remote sourcemeter was used to control the voltage to operate the single particle battery. During lithiation, the Ge particle was attached to the solid electrolyte and a bias voltage of -0.01 V was applied to the Ge particle. The all solid single particle battery provides the opportunity to observe the particle morphology change in operando. SEM images were taken every 5 s. To minimize the effect of the electron beam, we reduced the imaging current to about 40 pA, about 1% of the lithiation current. The TEM analysis was performed on a JEOL JEM2100F operated at 200 kV.
The TEM sample was prepared by a standard FIB lift-out procedure.
4
Rat Hippocampus 3D Reconstruction via FIB-SEM
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The original dataset used in this work (EM stack and 3D reconstructions) was previously published in [17] . The block was a gift from Graham Knott (BioEM imaging facility at EPFL, Lausanne, Switzerland). All procedures were performed according to the Swiss Federal Laws.
One P90 Sprague-Dawley rat was deeply anesthetized with isoflurane and transcardially perfused using 2% paraformaldehyde and 2.5% glutaraldehyde in PBS 0.1M. Coronal sections (100 µm) were obtained and washed in cacodylate buffer, followed by a post-fixation using osmium tetroxide and uranyl acetate. Finally, the sections were embedded in Durcupan. Regions of the hippocampus were dissected under a stereoscopic microscope, mounted onto a blank resin slab, and trimmed using an ultramicrotome (Laica Ultracut UC-7). Imaging was performed using an NVision 40 FIB-SEM (Carl Zeiss) with an acceleration voltage of 1.5 kV, a current of 350 pA, and a dwell time of 10 µs/pixel. Serial images were obtained using backscattered electrons and collected at a 6 nm/pixel magnification and 5 nm of milling depth between images.
One P90 Sprague-Dawley rat was deeply anesthetized with isoflurane and transcardially perfused using 2% paraformaldehyde and 2.5% glutaraldehyde in PBS 0.1M. Coronal sections (100 µm) were obtained and washed in cacodylate buffer, followed by a post-fixation using osmium tetroxide and uranyl acetate. Finally, the sections were embedded in Durcupan. Regions of the hippocampus were dissected under a stereoscopic microscope, mounted onto a blank resin slab, and trimmed using an ultramicrotome (Laica Ultracut UC-7). Imaging was performed using an NVision 40 FIB-SEM (Carl Zeiss) with an acceleration voltage of 1.5 kV, a current of 350 pA, and a dwell time of 10 µs/pixel. Serial images were obtained using backscattered electrons and collected at a 6 nm/pixel magnification and 5 nm of milling depth between images.
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