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Rhil 22

Manufactured by R&D Systems
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RhIL-22 is a recombinant human interleukin-22 protein. Interleukin-22 is a cytokine that plays a role in the regulation of immune responses and inflammatory processes.

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Lab products found in correlation

Hacat cells, by Thermo Fisher Scientific (1 mentions) Rhtnf α, by R&D Systems (1 mentions) Recombinant human il 17a, by R&D Systems (1 mentions) Rhil 1α, by R&D Systems (1 mentions) Hacat cell, by National Collection of Authenticated Cell Cultures (1 mentions) Boyden chamber, by Merck Group (1 mentions) Igg2a, by Genentech (1 mentions) Rmtnf α, by Thermo Fisher Scientific (1 mentions) 4 kda fitc dextran, by Merck Group (1 mentions) Millicell ers, by Merck Group (1 mentions) Rhifn γ, by R&D Systems (1 mentions) Rhtnf, by R&D Systems (1 mentions) Rhil 17a, by R&D Systems (1 mentions)

4 protocols using rhil 22

1

HaCaT Cell Cytokine Stimulation and GSDME Knockdown

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Human keratinocyte cell line, HaCaT cells, were obtained from China Center for Type Culture Collection (Wuhan, China). HaCaT cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) (both from Gibco, CA, USA) in 5% CO2 environment at 37 °C. HaCaT cells were treated with 10 ng/mL recombinant human (rh) IL-17A (R&D, Minneapolis, USA), 10 ng/mL rh OSM (R&D), 10 ng/mL rh TNF-α (R&D), 10 ng/mL rh IL22 (R&D), and 10 ng/mL rh IL1-α (R&D) in combination for 6, 12, 24, or 48 h. Transfection was performed according to previously described methods (45). For GSDME knockdown, HaCaT cells were transfected with pGLVH1/GFP + Puro lentivirus vector containing NC shRNA (shNC sequence: 5’-TTCTCCGAACGTGTCACGT-3’) or GSDME shRNA (shGSDME sequence: 5’-GCAGAAGTGTGTGATCTCTGA-3’) (GenePharma, Shanghai, China). Stable knockdown HaCaT cells were obtained and selected by puromycin incubation.
Long F., Wei X., Chen Y., Li M., Lian N., Yu S., Chen S., Yang Y., Li M., Gu H, & Chen X. (2024). Gasdermin E promotes translocation of p65 and c-jun into nucleus in keratinocytes for progression of psoriatic skin inflammation. Cell Death & Disease, 15(3), 180.
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2

Neutralizing Antibodies in IL-22 Study

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IL-22 neutralizing antibodies and IgG2a control were provided by Genentech (San Francisco, CA). rmIL-22 and rmTNF-α were from Peprotech (London, UK), rmIL-1a and rhIL-22 from R&D Systems (Minneapolis, MN), ELISA kits from Peprotech, R&D or Antigenix America and Boyden chambers from Millipore (Billerica, MA).
Giannou A.D., Kempski J., Shiri A.M., Lücke J., Zhang T., Zhao L., Zazara D.E., Cortesi F., Riecken K., Amezcua Vesely M.C., Low J.S., Xu H., Kaffe E., Garcia-Perez L., Agalioti T., Yamada Y., Jungraithmayr W., Zigmond E., Karstens K.F., Steglich B., Wagner J., Konczalla L., Carambia A., Schulze K., von Felden J., May P., Briukhovetska D., Bedke T., Brockmann L., Starzonek S., Lange T., Koch C., Riethdorf S., Pelczar P., Böttcher M., Sabihi M., Huber F.J., Reeh M., Grass J.K., Wahib R., Seese H., Stüben B.O., Fard-Aghaie M., Duprée A., Scognamiglio P., Plitzko G., Meiners J., Soukou S., Wittek A., Manthey C., Maroulis I.C., Arck P.C., Perez D., Gao B., Zarogiannis S.G., Strowig T., Pasqualini R., Arap W., Gosálvez J.S., Kobold S., Prinz I., Guse A.H., Tachezy M., Ghadban T., Heumann A., Li J., Melling N., Mann O., Izbicki J.R., Pantel K., Schumacher U., Lohse A.W., Flavell R.A., Gagliani N, & Huber S. (2023). Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22. Immunity, 56(1), 125-142.e12.
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3

Intestinal Epithelial Barrier Regulation

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T84 cells (human colonic adenocarcinoma epithelial cell line) were grown as a monolayer in Transwells (Corning, New York, NY) until differentiated into columnar epithelial cells. Before experiments, T84 cell transepithelial electrical resistance (TEER) was confirmed to be >1000 U/cm 2 with a voltmeter and chopstick electrodes (Millicell-ERS; Millipore, Billerica, MA). T84 cells were incubated with Tr1 or FOXP3positive Treg cell culture supernatants (from 4-day activated cultures) or 7 ng/mL rhIL22 (R&D Systems) for 60 minutes before addition of 10 ng/mL rh tumor necrosis factor (TNF) (R&D Systems). In some experiments, neutralizing anti-IL22 mAb (Invitrogen, #16-7222-85) was added to cultures at a 400:1 ratio to IL22. After 24 hours, TEER was measured and reported as a percentage of the pretreatment value. FITC-4-kDa dextran (Sigma-Aldrich) leakage through the T84 cell monolayer was measured and reported as the ratio of fluorescence in basolateral to apical supernatants. Experiments were performed with technical triplicates.
Cook L., Stahl M., Han X., Nazli A., MacDonald K.N., Wong M.Q., Tsai K., Dizzell S., Jacobson K., Bressler B., Kaushic C., Vallance B.A., Steiner T.S, & Levings M.K. (2019). Suppressive and Gut-Reparative Functions of Human Type 1 T Regulatory Cells. Gastroenterology, 157(6).
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4

Keratinocyte Cytokine Response Assay

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Keratinocytes were isolated from skin biopsy specimens, as previously described. 21 Ten thousand keratinocytes at passage 3 or 4 were plated in Abbreviations used HBD2: Human b-defensin 2 MX1: Myxovirus 1 NLP: Never-lesional skin from patients with psoriasis pDC: Plasmacytoid dendritic cell rh: Recombinant human 48-well plates. At 100% confluence, cultures were exposed to recombinant human (rh) IL-17A (5 ng/mL), rhIL-22 (5 ng/mL), rhTNF (5 ng/mL), rhIFN-a (5 U/mL), or rhIFN-g (5 ng/mL) (all from R&D Systems, Minneapolis, Minn) for 16 hours.
Gallais Sérézal I., Hoffer E., Ignatov B., Martini E., Zitti B., Ehrström M, & Eidsmo L. (2019). A skewed pool of resident T cells triggers psoriasis-associated tissue responses in never-lesional skin from patients with psoriasis. The Journal of allergy and clinical immunology, 143(4).
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