Whole blood was collected into heparinized tubes (365985, BD Biosciences, USA). Post-collection, the samples were centrifuged at 1000–2000 g for 10 min using a centrifuge at 4°C. Promptly after centrifugation, the supernatant (serum) was transferred to a sterile Eppendorf tube and preserved at 2°C–8°C throughout the experimentation. For further processing, samples were diluted and then incubated with RIPA lysis buffer for a duration of 30 min. Subsequent analysis was conducted in accordance with the manufacturer’s protocol for the Rat TNF-alpha DuoSet (DY510-05, R&D Systems, USA)
Rat tnf alpha duoset
Manufactured by R&D Systems
Sourced in United States
The Rat TNF-alpha DuoSet is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) used for the measurement of rat tumor necrosis factor alpha (TNF-alpha) in cell culture supernatants, serum, and plasma. The DuoSet provides the basic components required for the development of an ELISA to detect and quantify rat TNF-alpha.
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Lab products found in correlation
2 protocols using rat tnf alpha duoset
1
Rat TNF-alpha Quantification Protocol
2
Measurement of Mouse and Rat TNF-α
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Mouse TNF-alpha DuoSet (R&D Systems, Minneapolis, MN, USA) and rat TNF-alpha DuoSet (R&D Systems, Minneapolis, MN, USA) were used to measure TNF-α levels in culture media and plasma according to the manufacturer’s instructions.
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