LX-2 cells were co-transfected with the pGL6-miR‐based luciferase reporter plasmids (Beyotime, Beijing, China) containing wild‐type (WT) or mutant (MT) 3ʹ-UTR of Smad4, together with miR-618 agomir or miR-618 agomir-control using Lipofectamine® 2000. The Dual Luciferase Reporter Assay System (Beyotime Institute of Biotechnology, Beijing, China) was used to detect luciferase activity in LX-2 cell lysates [30 (link)].
Pgl6 mir based luciferase reporter plasmids
Manufactured by Beyotime
Sourced in China
The PGL6-miR‐based luciferase reporter plasmids are designed for the detection and measurement of microRNA (miRNA) activity. These plasmids contain a luciferase reporter gene that is regulated by a specific miRNA-binding site, allowing for the quantification of miRNA expression levels in cells.
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Lab products found in correlation
2 protocols using pgl6 mir based luciferase reporter plasmids
1
Validating miR-618 Target in HSCs
2
Evaluating PDHX 3'UTR Regulation by miR-181b-5p
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The pGL6-miR–based luciferase reporter plasmids (Beyotime, Beijing, China) containing wild‐type PDHX 3′UTR (pGL6 3′UTR‐wt) or PDHX mutated (pGL6 3′UTR‐mut) at the putative miR‐181b-5p binding sites were designed. Next, pGL6 3′UTR‐wt or pGL6 3′UTR‐mut, together with either agomir-control or miR‐181b-5p agomir were transfected into GBC cells with Lipofectamine® 2000 for 48 h. Finally, luciferase activity in cell lysates was detected with the Dual Luciferase Reporter Assay System (Beyotime). The renilla luciferase activity worked as an internal control.
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