Balb c ifn γ mice

Adult 6- to 8-week-old BALB/c and C57BL/6 mice were purchased from Charles River Laboratories through a contract with the National Cancer Institute. BALB/c IFN-γ^−/− mice were obtained from Jackson Laboratories (Bar Harbor, ME). C57BL/6 mice with macrophages insensitive to IFN-γ (MIIG) were previously generated at Cincinnati Children’s Hospital Medical Center (30 (link)). TβRIIf/f-Cre mice were generated by crossing Floxed TβRII (TβRIIf/f) and Ubc-CreERT2 (Cre) mice (14 (link)). To induce conditional deletion of TβRII, mice were injected intraperitoneally (i.p.) with 2 mg of tamoxifen (Sigma-Aldrich) in corn oil (Sigma-Aldrich) once daily for five consecutive days. Mice were treated with tamoxifen prior to intranasal (i.n.) allergen challenge. Animal care and experimental protocols were in accordance with the NIH Guide for the Care and Use of Laboratory Animals (81 ) and were approved by the Institutional Animal Care and Use Committee at Albany Medical College (protocol number 17-03006).