Eclipse te2000 u microscope

Animal protocols were approved and conducted in accordance with Yale Animal Resources Center and Institutional Animal Care and Use Committee regulations. Pdk1^fl/fl; Pax8^rtTA; TetO‐Cre mice and their induction regimen were previously described (Ma et al., 2013 (link)). Experiments were conducted on males. Retro‐orbital blood was collected from anesthetized mice for plasma BUN measurements (O’Brien Kidney Center at Yale). Upon sacrifice, kidney and body weights were recorded. Right kidneys were harvested and fixed in 4% PFA for histological analysis while contralateral kidneys were snap‐frozen. Images of whole‐kidney sections were obtained with a Nikon Eclipse TE2000‐U microscope under the control of MetaMorph software (Universal Imaging).

Bacillus anthracis cells were visualized using a Nikon Eclipse TE2000-U microscope and images were captured using MetaMorph version 6.2r6 (Universal Imaging Corporation). Phase contrast microscopy was used to visualize sporulating cells. India ink (Becton Dickinson Microbiology Systems, Sparks, MD, United States) exclusion methods were employed by mixing 7 μl culture and 3 μl India ink followed by wet-mounting 5 μl onto a glass slide for visualization (Breakwell et al., 2009 (link)). DIC imaging was used to visualize capsule.

After the differentiation process, H9c2 cells grown on glass-bottom dishes were incubated with TMRM (100 nM), Hoechst 33342 (1 mg/ml) or calcein-AM (300 nM) at 37°C in the dark. Media was replaced by warm Krebs buffer (1 mM CaCl₂; 132 mM NaCl; 4 mM KCl; 1.2 mM Na₂HPO₄; 1.4 mM MgCl₂; 6 mM glucose; 10 mM HEPES, pH 7.4) supplemented with 10% FBS. Epifluorescence microscopy images were obtained using a Nikon Eclipse TE2000U microscope and were acquired using Metamorph software (Universal Imaging, Downingtoen, PA).