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Csb e07433r

Manufactured by Cusabio
Sourced in China

The CSB‐E07433r is a laboratory product manufactured by Cusabio. It is designed for use in scientific research and laboratory applications. The core function of this product is to provide a specific tool or component for conducting experiments and analyses. No further details on the intended use or performance of this product can be provided in an unbiased and factual manner.

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2 protocols using csb e07433r

1

Plasma Biomarker Quantification Protocol

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Blood samples were collected in blood collection tubes coated with K2EDTA (BD, Cat# 36 784), and centrifuged for 15 min at 4 °C, 12 000 × g. Plasma GLP‐1, ghrelin, leptin, and LPS levels were determined by using corresponding ELISA kits CSB‐E08117r, CSB‐E09816 and CSB‐E07433r, and CSB‐E14247r (CUSABIO), respectively, according to the manufacturer's instructions.
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2

Serum Biomarker Profiling in Rats

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The blood was taken by heart puncture, and serum was immediately separated through centrifugation (3000 rpm for 10 min) and kept under −80 °C until analysis. The concentrations of Tumor Necrosis Factor α (TNF-α), Interluekin-6 (IL-6), Free fatty acids (FFA), insulin, and leptin in serum were measured using rat ELISA kits (CSB-E11987r, CSB-E04640r, CSB-E08770r, CSB-E05070r and CSB-E07433r, Cusabio Biotech Co., Ltd, China). Glucose, Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT), Triglyceride and Cholesterol were measured using automatic measuring analyzer (Roche Diagnostic).
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