Penicillin streptomycin pen strep broad spectrum antibiotic cocktail

Collagenase type I, penicillin/streptomycin (Pen/Strep) broad-spectrum antibiotic cocktail, trypsin-EDTA (0.25%), fetal bovine serum (FBS), human insulin and Dulbecco’s Modified Eagle’s Medium (DMEM) were purchased from Gibco/Invitrogen (Carlsbad, CA, USA). VersaLyse^TM was purchased from Beckman Coulter (Miami, FL, USA). Dexamethasone, 3-isobutyl-methylxanthine, Nile Red (NR) and indomethacin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Vybrant® DyeCycle^TM Violet was purchased from Thermo Fisher Scientific/Life Technologies (Eugene, OR, USA). The following mouse anti-human monoclonal antibodies were purchased from Biolegend (San Diego, CA, USA): CD14-APC Cy7 (Clone M5E2), CD31-PE Cy7 (Clone WM-59), CD36-APC (Clone 5-271), CD73-FITC (Clone AD2), CD44-APC Cy7 (Clone IM7) and CD105-PE (Clone 42A3). Mouse anti-human CD45-Krome Orange (Clone J.33), CD90-PE-Cy5 (Clone Thy-1), CD34-PE Cy7 (Clone 581), and the viability dye, 7-aminoactinomycin D (7-AAD) were purchased from Immunotech/Beckman Coulter (Marseille, France).

Collagenase type I, penicillin/streptomycin (Pen/Strep) broad spectrum antibiotic cocktail, trypsin-EDTA (0.25%), PBS, α-MEM, and DMEM culture media were purchased from Gibco/Invitrogen (Carlsbad, CA). VersaLyse was purchased from Beckman Coulter (Miami, FL). FBS was purchased from Lonza (Basel, Switzerland). Dexamethasone, 3-isobutyl-methylxanthine, indomethacin, and human insulin were purchased from Sigma-Aldrich (St. Louis, MO). The 4′,6-diamino-2-phenylindole dihydrochloride (DAPI), Vybrant DyeCycle Ruby, NR, and BDP were purchased from Thermo Fisher Scientific/Life Technologies (Eugene, OR). Mouse anti-human CD36 conjugated to the fluorochrome, allophycocyanin (APC) (clone 5-271), was purchased from Biolegend (San Diego, CA).