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Direct camp colorimetric eia kit

Manufactured by Enzo Life Sciences
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Sourced in United Kingdom

The Direct cAMP colorimetric (EIA) kit is a laboratory equipment product that measures the concentration of cyclic adenosine monophosphate (cAMP) in a sample. It utilizes an enzyme immunoassay (EIA) technique to quantify the cAMP levels.

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EIA kit (41 citations) Direct cAMP EIA Kit (2 citations)

2 protocols using direct camp colorimetric eia kit

1

Quantifying Intracellular cAMP Levels

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Cultures of 2-day-old liquid mycelial were harvested, frozen in liquid nitrogen and lyophilized more than 6 h. Intracellular cAMP extraction was followed as previously described (Liu et al., 2007 (link)). The cAMP levels were quantified according to the Direct cAMP colorimetric (EIA) kit (Enzo Life Sciences, Exeter, UK). The experiment was conducted with technical triplicates for each strain, and was repeated thrice.
Yang K., Qin Q., Liu Y., Zhang L., Liang L., Lan H., Chen C., You Y., Zhang F, & Wang S. (2016). Adenylate Cyclase AcyA Regulates Development, Aflatoxin Biosynthesis and Fungal Virulence in Aspergillus flavus. Frontiers in Cellular and Infection Microbiology, 6, 190.
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2

Intracellular cAMP and PKA Assay in A. flavus

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To assay the intracellular cAMP levels in the A. flavus strains, the liquid shaken mycelial were harvested after 48 h of inoculation and lyophilized overnight. The extraction of cAMP was conducted according to a previously-described method [24 (link),38 (link)]. The Direct cAMP colorimetric (EIA) kit (Enzo Life Sciences, Exeter, U.K.) was used to quantify the cAMP levels.
The free dry mycelium was used for total protein extraction following the protocol as previously described [24 (link),39 (link)]. To measure the PKA activities, the PepTag® Assay for Non-Radioactive Detection of the cAMP-Dependent Protein Kinase kit (Promega, Madison, WI, USA) was utilized.
Liu Y., Yang K., Qin Q., Lin G., Hu T., Xu Z, & Wang S. (2018). G Protein α Subunit GpaB is Required for Asexual Development, Aflatoxin Biosynthesis and Pathogenicity by Regulating cAMP Signaling in Aspergillus flavus. Toxins, 10(3), 117.
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