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Nh4cl

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Sourced in China

NH4Cl is a chemical compound that consists of ammonium and chloride ions. It is a white, crystalline solid that is commonly used as a laboratory reagent and in various industrial applications.

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Lab products found in correlation

Mg132, by MedChemExpress (2 mentions) Cycloheximide (chx), by MedChemExpress (1 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Polyinosinic polycytidylic acid poly 1 c, by Merck Group (1 mentions) Chloroquine, by Cell Signaling Technology (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions) Actinomycin d, by MedChemExpress (1 mentions) Nh4cl, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Jetpei macrophage, by Polyplus Transfection (1 mentions) Jetprime, by Polyplus Transfection (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Z vad fmk, by MedChemExpress (1 mentions)

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Ammonium chloride (NH4Cl), (2 citations)

3 protocols using nh4cl

1

Antibody Characterization for Crucian Carp Immunity

Cited 1 time
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Anti-FLAG (1:5000, catalog no. 14793S), anti-HA (1:5000, catalog no. 3724S), and anti-β-actin (1:5000, catalog number: 4967S) were purchased from Cell Signaling Technology (Danvers, MA) and ABclonal Technology (Wuhan, China), respectively. Anti-TBK1 Ab was generated by immunization of rabbits with a purified peptide corresponding to 360–550 aa of zebrafish TBK1. Anti-CaIRF7 and anti-CaSTAT1 Abs were generated by immunization of rabbits with purified full length peptides of crucian carp IRF7 and STAT1.14 (link),49 (link)
MG132, NH4Cl, Cycloheximide (CHX) and Actinomycin D (ActD) were purchased from MedChemExpress (Monmouth Junction, NJ). Chloroquine was from Cell Signaling Technology (Danvers, MA). Polyinosinic-polycytidylic acid [poly(I:C)] was from Sigma-Aldrich (St. Louis, MO).
Qu Z.L., Gong X.Y., An L.L., Sun H.Y., Guo W.H., Luan H.Y., Wu M.Y., Dan C., Gui J.F, & Zhang Y.B. (2024). Genome editing of FTR42 improves zebrafish survival against virus infection by enhancing IFN immunity. iScience, 27(4), 109497.
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2

Amyloid-beta Peptide Utilization in Antiviral Studies

Cited 1 time
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Aβ peptides 1–42 are a major pathological factor of senile plaques in AD [16 (link), 17 (link)]. Accordingly, we utilized Aβ1–42 as a test subject to perform our study.
Synthetic Aβ1–42 peptides were obtained from Anaspec (Fremont, CA. USA). Dried peptides were solubilized in 150 mM NaCl prior to experimentation. Aβ1–42 was used at 30 µg/mL concentration for the viral life-cycle experiment and 20 µg/mL for the antiviral activity test or pull-down experiment.
Pirodavir was purchased from Biochempartner (Shanghai, China), and NH4Cl was bought from MedChemExpress (USA). Stock solutions of pirodavir (10 mM) and NH4Cl (100 mM) were prepared in dimethyl sulfoxide (Sigma–Aldrich, Carlsbad, CA, USA), respectively. RBV stock solutions (2 mg/mL) were dissolved in a cell culture medium.
Zhong M., Wang H., Yan H., Wu S., Wang K., Yang L., Cui B., Wu M, & Li Y. (2022). Effects and mechanism of Aβ1−42 on EV-A71 replication. Virology Journal, 19, 151.
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3

Antibody Characterization and Cellular Treatments

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The anti-FLAG (F7425), anti-Myc (M4439), β-Actin (A5441), anti-GSDMD (SAB4200798) was purchased from Sigma-Aldrich Co., Ltd (Shanghai, China). The anti-FoxJ1 Polyclonal Antibody was purchased from Abbkine Scientific Co., Ltd (Wuhan, China). Horseradish peroxidase HRP-Goat anti-Rabbit IgG (H+L) (SA00001-2), HRP-Goat anti-Mouse IgG (H+L) (SA00001-1) was purchased from Lanzhou Lihe Biotechnology (Lanzhou, China).
MG132, chloroquine (CQ), 3-MA, NH4Cl, and Z-VAD-FMK were purchased from MedChemExpress (New Jersey, USA). Lipofectamine2000 transfection reagent (11668019) was purchased from Thermo Fisher Scientific (Massachusetts, USA). jetPRIME and jetPEI-Macrophage were purchased from Polyplus Transfection (Strasbourg, France).
Ma C., Li S., Yang F., Cao W., Liu H., Feng T., Zhang K., Zhu Z., Liu X., Hu Y, & Zheng H. (2022). FoxJ1 inhibits African swine fever virus replication and viral S273R protein decreases the expression of FoxJ1 to impair its antiviral effect. Virologica Sinica, 37(3), 445-454.
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