ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1 .
Mouse monoclonal anti flag m2 f 3165
Manufactured by Merck Group
The Mouse monoclonal anti-FLAG (M2) (F-3165) is a laboratory reagent intended for use in various research applications. It is a mouse-derived monoclonal antibody that specifically recognizes the FLAG epitope, a small peptide tag commonly used to label and identify recombinant proteins.
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Lab products found in correlation
3 protocols using mouse monoclonal anti flag m2 f 3165
1
Chromatin Immunoprecipitation (ChIP) Analysis
2
Antibodies for Synaptojanin1 Characterization
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Five anti-Synaptojanin1 antibodies were used in this study (Supplementary Table 1 , online resource). Rabbit polyclonal anti-SYNJ1 (HPA011916) was purchased from Sigma. Mouse monoclonal anti-SYNJ1 (BD612249, sc-32,770, TA309245) antibodies were purchased from BD transduction, Santa Cruz Biotechnology and Origene, respectively. Rabbit polyclonal anti-SYNJ1 ab19904 antibody was purchased from Abcam. Mouse monoclonal anti-Flag M2 (F3165), and mouse monoclonal anti-actin antibodies (A5441) were purchased from Sigma. Mouse monoclonal anti-tau antibody recognizing pSer396/Ser404 tau (PHF1) was kindly provided by Dr. Peter Davies (Albert Einstein College of Medicine, NY). Mouse monoclonal anti-Synaptophysin (SY38) was purchased from abcam.
3
Chromatin Immunoprecipitation (ChIP) Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChIP analysis was performed using Magna ChIP kits (Millipore) according to the manufacturer’s instruction. The following antibodies were used in ChIP: mouse monoclonal anti-FLAG (M2) (F-3165, Sigma-Aldrich) (for FLAG-cyclin D1), mouse anti-G9a (clonal A8620A) (PP-A8620A-00) (R&D System), rabbit anti-G9a (clonal C6H3) (Cell signaling), and mouse anti-H3K9me2 (ab1220) (Abcam Inc.). For a negative control, mouse IgG was from the Magna ChIP kits (Millipore). SYBR Green PCR kit (Invitrogen) was used in the ChIP-real time PCR (ChIP-qPCR). The primers used in ChIP-qPCR are shown in the Supplemental Table 1 .
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